Anti-CD45/PTPRC Antibody
Boster
catalog: PA1596

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section

Anti-CD45 antibody, PA1596, Western blotting. All lanes: Anti CD45 (PA1596) at 0.5ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40ug. Lane 2: CEM Whole Cell Lysate at 40ug. Predicted bind size: 147KD. Observed bind size: 147KD

Anti-CD45 antibody, PA1596, IHC(P). IHC(P): Human Tonsil Tissue

Anti-CD45 antibody, PA1596, ICC. ICC: JURKAT Cell
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CD45/PTPRC Antibody Picoband™
Boster
catalog: PB9096

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Anti-CD45 Picoband antibody, PB9096-1.jpg. All lanes: Anti CD45 (PB9096) at 0.5ug/ml. WB: Recombinant Human CD45 Protein 0.5ng. Predicted bind size: 47KD. Observed bind size: 47KD

Anti-CD45 Picoband antibody, PB9096-2.jpg. All lanes: Anti CD45 (PB9096) at 0.5ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40ug. Lane 2: RAJI Whole Cell Lysate at 40ug. Predicted bind size: 147KD. Observed bind size: 147KD

Anti-CD45 Picoband antibody, PB9096-3.JPG. IHC(P): Human Tonsil Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CD45/PTPRC Antibody Picoband™
Boster
catalog: PB9785

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Figure 1. Western blot analysis of CD45 using anti-CD45 antibody (PB9785). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: JURKAT Whole Cell Lysate,. Lane 2: HL-60 Whole Cell Lysate,. Lane 3: K562 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD45 antigen affinity purified polyclonal antibody (Catalog # PB9785) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD45 at approximately 220KD. The expected band size for CD45 is at 220KD.

Figure 2. IHC analysis of CD45 using anti-CD45 antibody (PB9785). CD45 was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD45 Antibody (PB9785) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IF analysis of CD68 and CD45 using anti-CD68 antibody (M00602) and anti-CD45 antibody (PB9785) . CD68 and CD45 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL mouse anti- CD68 Antibody (M00602) and rabbit anti CD45 Antibody(PB9785) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) and Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The tissue section was developed using Cy3 Conjugated Avidin (BA1037). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CD45 PTPRC Rabbit Monoclonal Antibody
Boster
catalog: M00555-1

domestic rabbit monoclonal (CBI-16)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry

Immunohistochemical analysis of paraffin-embedded human spleen, using CD45 Antibody (M00555-1). PTPRC was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PTPRC Antibody (M00555-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of CD45 expression in Jurkat cell lysate (M00555-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPRC monoclonal antibody (Catalog # M00555-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPRC
quantity: 100 µl
price: 315 USD
to the supplier

Anti-CD45/PTPRC Antibody Picoband™
Boster
catalog: A00555-2

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA

Figure 1. Western blot analysis of CD45 using anti- CD45 antibody (A00555-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, . Lane 2: mouse thymus tissue lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CD45 antigen affinity purified polyclonal antibody (Catalog # A00555-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD45 at approximately 200KD. The expected band size for CD45 is at 147KD.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Human CD45 DyLight® 488 conjugated PTPRC Antibody
Boster
catalog: PB9096-Dyl488

domestic rabbit polyclonal
reactivity: human
conjugate: DyLight488
application: immunocytochemistry, flow cytometry

Figure 2. IF analysis of CD45 using anti- CD45 antibody (PB9096-Dyl488) . CD45 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti- CD45 Antibody (PB9096-Dyl488) overnight at 4°C. DyLight?488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Figure 3. IF analysis of CD45 using anti- CD45 antibody (PB9096-Dyl488) . CD45 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti- CD45 Antibody (PB9096-Dyl488) overnight at 4°C. DyLight?488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
quantity: 50 ug/vial
price: 415 USD
to the supplier

Anti-CD45/PTPRC Antibody Picoband™
Boster
catalog: A00555-3

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-CD45 Antibody Picoband™ (monoclonal, 2H3)
Boster
catalog: M00555-5

mouse monoclonal (2H3)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 µg/vial
price: 315 USD
to the supplier

Anti-CD45 Antibody Picoband™ (monoclonal, 3H6)
Boster
catalog: M00555-6

mouse monoclonal (3H6)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 µg/vial
price: 315 USD
to the supplier