Anti-SHP2/PTPN11 Antibody
Boster
catalog: PA1114

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Anti-SHP2 antibody, PA1114, Western blotting. WB: JURKAT Cell Lysate

Anti-SHP2 antibody, PA1114, IHC(P). IHC(P): Human Thyroid Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-SHP2/PTPN11 Antibody
Boster
catalog: PA1860

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Figure 1. Western blot analysis of SHP2 using anti- SHP2 antibody (PA1860). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Heart Tissue Lysate,. Lane 2: Rat Skeletal Muscle Tissue Lysate,. Lane 3: Rat Brain Tissue Lysate,. Lane 4: HELA Whole Cell Lysate,. Lane 5: A549 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- SHP2 antigen affinity purified polyclonal antibody (Catalog # PA1860) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHP2 at approximately 68KD. The expected band size for SHP2 is at 68KD.

Figure 2. Western blot analysis of SHP2 using anti- SHP2 antibody (PA1860). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate,. Lane 2: Rat Kidney Tissue Lysate,. Lane 3: Rat Heart Tissue Lysate,. Lane 4: Rat Skeletal Muscle Tissue Lysate,. Lane 5: A431 Whole Cell Lysate,. Lane 6: JURKAT Whole Cell Lysate,. Lane 7: HELA Whole Cell Lysate,. Lane 8: U87 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- SHP2 antigen affinity purified polyclonal antibody (Catalog # PA1860) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHP2 at approximately 68KD. The expected band size for SHP2 is at 68KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-SHP2/PTPN11 Antibody Picoband™
Boster
catalog: PB9675

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Anti- SHP2 Picoband antibody, PB9675, Western blotting. All lanes: Anti SHP2 (PB9675) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: Mouse Brain Tissue Lysate at 50ug. Lane 3: Rat Cardiac Muscle Tissue Lysate at 50ug. Lane 4: Mouse Kidney Tissue Lysate at 50ug. Lane 5: HELA Whole Cell Lysate at 40ug. Lane 6: SW620 Whole Cell Lysate at 40ug. Lane 7: HEPG2 Whole Cell Lysate at 40ug. Lane 8: JURKAT Whole Cell Lysate at 40ug. Predicted bind size: 68KD. Observed bind size: 68KD

Anti- SHP2 Picoband antibody, PB9675,IHC(P). IHC(P): Mouse Cardiac Muscle Tissue

Anti- SHP2 Picoband antibody, PB9675,IHC(P). IHC(P): Rat Skeletal Muscle Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-SHP2/PTPN11 Antibody Picoband™ (monoclonal, 2E6)
Boster
catalog: M00150-2

mouse monoclonal (2000000)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Boster Kit Box
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Phospho-SHP2 (Y542) PTPN11 Rabbit Monoclonal Antibody
Boster
catalog: P00150

domestic rabbit monoclonal (FAB-16)
reactivity: human, mouse
application: western blot, immunocytochemistry, immunoprecipitation

Western blot analysis of Phospho-SHP2 (Y542) expression in (1) NIH/3T3 cell lysates; (2) NIH/3T3 cell lysates treated with PDGF (P00150). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN11 monoclonal antibody (Catalog # P00150) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN11
quantity: 100 µl
price: 315 USD
to the supplier

Anti-SHP2 PTPN11 Rabbit Monoclonal Antibody
Boster
catalog: M00150-1

domestic rabbit monoclonal (GBO-16)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation

Western blot analysis of SHP2 expression in A431 cell lysate (M00150-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN11 monoclonal antibody (Catalog # M00150-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN11
quantity: 100 µl
price: 315 USD
to the supplier

Anti-SHP2 PTPN11 Rabbit Monoclonal Antibody
Boster
catalog: M00150

domestic rabbit monoclonal (GCA-16)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of SHP2 expression in Jurkat cell lysate (M00150). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN11 monoclonal antibody (Catalog # M00150) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTPN11
quantity: 100 µl
price: 315 USD
to the supplier