Anti-COX2/Cyclooxygenase 2/PTGS2 Antibody Picoband™
Boster
catalog: A00084

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Figure 1. Western blot analysis of PTGS2 using anti- PTGS2 antibody (A00084). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, . Lane 2: mouse kidney tissue lysates, . Lane 3: HELA whole Cell lysates, . Lane 4: 22RV1 whole Cell lysates, . Lane 5: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PTGS2 antigen affinity purified polyclonal antibody (Catalog # A00084) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGS2 at approximately 75KD. The expected band size for PTGS2 is at 69KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-COX2/Cyclooxygenase 2/PTGS2 Antibody
Boster
catalog: PA1725

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Anti-COX2/Cyclooxygenase 2 antibody, PA1725, Western blotting. All lanes: Anti COX2/Cyclooxygenase 2 (PA1725) at 0.5ug/ml. Lane 1: Human Placenta Tissue Lysate at 50ug. Lane 2: COLO320 Whole Cell Lysate at 40ug. Lane 3: HELA Whole Cell Lysate at 40ug. Lane 4: PANC Whole Cell Lysate at 40ug. Lane 5: SKOV Whole Cell Lysate at 40ug. Predicted bind size: 69KD. Observed bind size: 69KD

Anti-COX2/Cyclooxygenase 2 antibody, PA1725, IHC(P). IHC(P): Human Intestinal Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-MUC1 PTGS2 Antibody Picoband™
Boster
catalog: PB9145

domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry

Anti-MUC1 Picoband antibody, PB9145-1.JPG. IHC(P): Human Ovary Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-MyD88 PTGS2 Antibody Picoband™
Boster
catalog: PB9148

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Figure 1. Western blot analysis of MYD88 using anti-MYD88 antibody (PB9148). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human MYD88 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody (Catalog # PB9148) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYD88 at approximately 49KD. The expected band size for MYD88 is at 49KD.

Figure 2. Western blot analysis of MYD88 using anti-MYD88 antibody (PB9148). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Cardiac Muscle Tissue Lysate. Lane 2: HELA Whole Cell Lysate. Lane 3: MCF Whole Cell Lysate. Lane 4: HEPG2 Whole Cell Lysate. Lane 5: JURKAT Whole Cell Lysate. Lane 6: RAJI Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody (Catalog # PB9148) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYD88 at approximately 33KD. The expected band size for MYD88 is at 33KD.

Figure 3. IHC analysis of MYD88 using anti-MYD88 antibody (PB9148). MYD88 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYD88 Antibody (PB9148) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-COX2 PTGS2 Rabbit Monoclonal Antibody
Boster
catalog: M00084

domestic rabbit monoclonal (EEC-16)
reactivity: human, mouse
application: western blot, immunocytochemistry

Western blot analysis of COX2 expression in RAW2647 cell lysate treated with LPS (M00084). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGS2 monoclonal antibody (Catalog # M00084) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGS2

IF analysis of immunocytochemical section of Hela cells using anti- COX2 antibody (M00084) . COX2 was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- COX2 Antibody (M00084) overnight at 4 C. DyLight®488 Conjugated Goat AntiRabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-COX2/Cyclooxygenase 2/PTGS2 Picoband™ Antibody
Boster
catalog: A00084-2

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, flow cytometry

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Cox-2 (T594) PTGS2 Antibody
Boster
catalog: A00084T594

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 100 ul
price: 512 USD
to the supplier

Anti-Cox2 PTGS2 Monoclonal Antibody
Boster
catalog: M00084-1

domestic rabbit monoclonal (AFHD-16)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation

quantity: 100 µl
price: 315 USD
to the supplier

Anti-COX-2 PTGS2 Rabbit Monoclonal Antibody, Clone#RM348
Boster
catalog: M00084-2

domestic rabbit monoclonal (RM348)
reactivity: human
application: western blot, immunohistochemistry

quantity: 100 uL
price: 399 USD
to the supplier

Anti-COX2 PTGS2 Monoclonal Antibody
Boster
catalog: M00084-3

mouse monoclonal (3D4)
reactivity: human, mouse, rat
application: immunohistochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-PTGS2 Antibody
Boster
catalog: M00084-4

mouse monoclonal (monoclonal)
reactivity: human
application: western blot

quantity: 50 ul
price: 170 USD
to the supplier