domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Anti-PKR antibody, PA2025, Western blotting. Recombinant Protein Detection Source: E.coli derived -recombinant Human EIF2AK2, 35.0KD (162aa tag+ M1-K154). Lane 1: Recombinant Human EIF2AK2 Protein 10ng . Lane 2: Recombinant Human EIF2AK2 Protein 5ng . Lane 3: Recombinant Human EIF2AK2 Protein 2.5ng . Lane 4: Recombinant Human EIF2AK2 Protein 1.25ng
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry
reactivity: human
application: western blot, immunocytochemistry
domestic rabbit monoclonal (BIC-5)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
Western blot analysis of PKR phosphorylation expression in HeLa cell lysate treated with Calyculin A and TNF-alpha (P01384). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2AK2 monoclonal antibody (Catalog # P01384) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2AK2
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit monoclonal (FCH-5)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
Western blot analysis of PKR expression in MCF-7 cell lysate (M01384). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2AK2 monoclonal antibody (Catalog # M01384) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2AK2
quantity: 100 µl
price: 315 USD
to the supplier