domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
Anti-MEK3 antibody, PA1377, Western blotting. Lane 1: Rat Spleen Tissue Lysate. Lane 2: Rat Thymus Tissue Lysate. Lane 3: Rat Skeletal Muscle Tissue Lysate. Lane 4: Rat Kidney Tissue Lysate. Lane 5: MCF-7 Cell Lysate. Lane 6: HELA Cell Lysate. Lane 7: RAJI Cell Lysate. Lane 8: CEM Cell Lysate. Lane 9: COLO320 Cell Lysate
Anti-MEK3 antibody, PA1377, IHC(P). IHC(P): Human Mammary Cancer Tissue
Figure 3. Western blot analysis of MEK3 using anti-MEK3 antibody (PA1377). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse HEPA1-6 whole cell lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK3 antigen affinity purified polyclonal antibody (Catalog # PA1377) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK3 at approximately 39KD. The expected band size for MEK3 is at 39KD.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
Anti- MEK3 Picoband antibody, PB9763, Western blotting. All lanes: Anti MEK3 (PB9763) at 0.5ug/ml. Lane 1: Mouse Spleen Tissue Lysate at 50ug. Lane 2: HELA Whole Cell Lysate at 40ug. Predicted bind size: 40KD. Observed bind size: 40KD
Anti- MEK3 Picoband antibody, PB9763, IHC(P). IHC(P): Mouse Skeletal Muscle Tissue
Anti- MEK3 Picoband antibody, PB9763, IHC(P). IHC(P): Rat Skeletal Muscle Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Anti-MEK3 antibody, PA1377-1, All Western blotting. All lanes: Anti-MAP2K3(PA1377-1) at 0.5ug/ml. Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug. Lane 2: Rat Kidney Tissue Lysate at 40ug. Lane 3: Rat Spleen Tissue Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Lane 5: A549 Whole Cell Lysate at 40ug. Lane 6: JURKAT Whole Cell Lysate at 40ug. Lane 7: CEM Whole Cell Lysate at 40ug. Predicted bind size: 39KD. Observed bind size: 39KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (HDA-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
Western blot analysis of MEK3/MEK6 expression in HeLa cell lysate (M02916). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 monoclonal antibody (Catalog # M02916) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit monoclonal (HDB-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
Western blot analysis of MEK3 expression in HepG2 cell lysate (M02916-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 monoclonal antibody (Catalog # M02916-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K3
quantity: 100 µl
price: 315 USD
to the supplier