Anti-MEK1/MAP2K1 Antibody
Boster
catalog: PA1376

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Anti-MEK1 antibody, PA1376, Western blotting. Lane 1: Rat Skeletal Muscle Tissue Lysate. Lane 2: Rat Kidney Tissue Lysate. Lane 3: CEM Cell Lysate. Lane 4: COLO20 Cell Lysate

Anti-MEK1 antibody, PA1376, IHC(P). IHC(P): Human Lung Cancer Tissue

Anti-MEK1 antibody, PA1376, IHC(P). IHC(P): Human Mammary Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-MEK1/2 MAP2K1 Rabbit Monoclonal Antibody
Boster
catalog: M00292-1

domestic rabbit monoclonal (BOA-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Immunohistochemical analysis of paraffin-embedded human kidney, using MEK1/2 Antibody(M00292-1). MAP2K1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP2K1 Antibody (M00292-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of MEK1/2 expression in (1) 293T cell lysate; (2) A549 cell lysate (M00292-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # M00292-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-MEK1 MAP2K1 Rabbit Monoclonal Antibody
Boster
catalog: M00292-2

domestic rabbit monoclonal (IF-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of MEK1 expression in (1) A431 cell lysate;(2) HeLa cell lysate (M00292-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # M00292-2) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-MEK1 MAP2K1 Rabbit Monoclonal Antibody
Boster
catalog: M00292

domestic rabbit monoclonal (DCG-13)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of MEK1 expression in A431 cell lysate (M00292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # M00292) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Phospho-MEK1 (S298) MAP2K1 Rabbit Monoclonal Antibody
Boster
catalog: P00292-3

domestic rabbit monoclonal (HBO-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of Phospho-MEK1 (S298) expression in (1) HeLa cell treated with LP lysate; (2) HeLa cell lysate (P00292-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # P00292-3) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Phospho-MEK1 (T292) MAP2K1 Rabbit Monoclonal Antibody
Boster
catalog: P00292-4

domestic rabbit monoclonal (HCG-13)
reactivity: human
application: western blot

Western blot analysis of MEK5 expression in (1) HeLa cell lysate; (2) HeLa cell treated with Nocodazole (P00292-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody (Catalog # P00292-4) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-MEK1/MAP2K1 Antibody Picoband™
Boster
catalog: A00292

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, flow cytometry

quantity: 100 µg/vial
price: 315 USD
to the supplier