Anti-MAPK8/9 Antibody
Boster
catalog: PA1407

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Anti-MAPK8/9 antibody, PA1407, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: Rat Thymus Tissue Lysate. Lane 3: MCF-7 Cell Lysate. Lane 4: HELA Cell Lysate. Lane 5: JURKAT Cell Lysate. Lane 6: MM231 Cell Lysate. Lane 7: CEM Cell Lysate

Anti-MAPK8/9 antibody, PA1407, IHC(P). IHC(P): Rat Intestine Tissue

Anti-MAPK8/9 antibody, PA1407, IHC(P). IHC(P): Human Intestinal Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-JNK1/MAPK8 Antibody
Boster
catalog: PA1892

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Anti-JNK1 antibody, PA1892, Western blotting. WB: HT1080 Cell Lysate
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-JNK1/JNK3 MAPK8 Rabbit Monoclonal Antibody
Boster
catalog: M02608

domestic rabbit monoclonal (ICC-13)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation

Western blot analysis of JNK1/JNK3 expression in HeLa cell lysate (M02608). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK8 monoclonal antibody (Catalog # M02608) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK8
quantity: 100 µl
price: 315 USD
to the supplier

Anti-JNK1/2/3 MAPK8 Rabbit Monoclonal Antibody
Boster
catalog: M02608-1

domestic rabbit monoclonal (DFI-13)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation

Western blot analysis of JNK1/2/3 Antibody expression in HeLa cell lysate (M02608-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK8 monoclonal antibody (Catalog # M02608-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK8
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Phospho-JNK1/2/3 (T183+T183+T221) MAPK8 Monoclonal Antibody
Boster
catalog: MP02608

domestic rabbit monoclonal (EAG-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Immunofluorescent analysis of NIH/3T3 cells treated with Anisomycin, using Phospho-JNK1/2/3 (T183+T183+T221) Antibody.

Figure 2. IHC analysis of MAPK8 using anti-MAPK8 antibody (MP02608). MAPK8 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAPK8 Antibody (MP02608) overnight at 4 C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of MAPK8 using anti-MAPK8 antibody (MP02608). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK8 antigen affinity purified polyclonal antibody (Catalog # MP02608) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MAPK8.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-JNK1/2/3 (T178) MAPK8 Antibody
Boster
catalog: A02608T178

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 100 ul
price: 512 USD
to the supplier

Anti-JNK1/2/3 (T183/Y185) MAPK8 Antibody
Boster
catalog: A02608T183Y185

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry

quantity: 100 ul
price: 512 USD
to the supplier

Anti-JNK1/2/3 (Y185) MAPK8 Antibody
Boster
catalog: A02608Y185

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: immunohistochemistry

quantity: 100 ul
price: 512 USD
to the supplier

Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody
Boster
catalog: A02608Y185-1

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry

quantity: 100 ul
price: 380 USD
to the supplier