Anti-Cytochrome P450 Reductase/POR Antibody
Boster
catalog: PA1952
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Anti-POR antibody, PA1952, Western blotting. Lane 1: Rat Ovary Tissue Lysate. Lane 2: Rat Lung Tissue Lysate. Lane 3: Rat Testis Tissue Lysate. Lane 4: Rat Spleen Tissue Lysate. Lane 5: A549 Cell Lysate . Lane 6: HELA Cell Lysate. Lane 7: SKOV Cell Lysate. Lane 8: MCF-7 Cell Lysate

Anti-POR antibody, PA1952, IHC(P). IHC(P): Human Placenta Tissue

Anti-POR antibody, PA1952, ICC. ICC: HELA Cell
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-Cytochrome P450 Reductase/POR Antibody Picoband™
Boster
catalog: PB9736
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Figure 1. Western blot analysis of POR using anti-POR antibody (PB9736). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate,. Lane 2: Human Placenta Tissue Lysate,. Lane 3: HEPG2 Whole Cell Lysate,. Lane 4: HEPA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POR antigen affinity purified polyclonal antibody (Catalog # PB9736) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POR at approximately 85KD. The expected band size for POR is at 77KD.

Figure 2. IHC analysis of POR using anti-POR antibody (PB9736). POR was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-POR Antibody (PB9736) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of POR using anti-POR antibody (PB9736). POR was detected in paraffin-embedded section of Rat Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-POR Antibody (PB9736) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-Human POR DyLight® 488 conjugated Antibody
Boster
catalog: A02166-Dyl488
domestic rabbit polyclonal
reactivity: human
conjugate: DyLight488
application: flow cytometry
quantity: 50 ug/vial
price: 415 USD
to the supplier
Anti-Human POR DyLight® 550 conjugated Antibody
Boster
catalog: A02166-Dyl550
domestic rabbit polyclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier
Anti-Cytochrome P450 Reductase Monoclonal Antibody
Boster
catalog: M02166
domestic rabbit monoclonal (AEFH-16)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
quantity: 100 µl
price: 315 USD
to the supplier
Anti-POR Antibody Picoband™ (monoclonal, 7F5)
Boster
catalog: M02166-2
mouse monoclonal (7F5)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 µg/vial
price: 315 USD
to the supplier