Anti-CD31 PECAM1 Antibody
Boster
catalog: A01513
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

CD31 was detected in paraffin-embedded sections of vascular endothelium in human colon tissues at 20x (B) and at 40x (C) using rabbit anti- CD31 Antigen Affinity purified polyclonal antibody (Catalog # A01513) at 4.0 ug/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).

Western blot analysis of CD31 expression in NIH3T3 whole cell lysates (lane 1), JURKAT whole cell lysates (lane 2) and HT-29 whole cell lysates (lane 3). CD31 at 80KD was detected using rabbit anti- CD31 Antigen Affinity purified polyclonal antibody (Catalog # A01513) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
quantity: 100 uL
price: 487 USD
to the supplier
Anti-CD31/PECAM1 Antibody Picoband™
Boster
catalog: PB9094
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of CD31 using anti-CD31 antibody (PB9094). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human CD31 protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31 antigen affinity purified polyclonal antibody (Catalog # PB9094) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD31 at approximately 39KD. The expected band size for CD31 is at 39KD.

Figure 2. Western blot analysis of CD31 using anti-CD31 antibody (PB9094). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HELA whole cell lysate,. lane 2: U937 whole cell lysate,. lane 3: MM231 whole cell lysate,. lane 4: JURKAT whole cell lysate,. lane 5: RAJI whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31 antigen affinity purified polyclonal antibody (Catalog # PB9094) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD31 at approximately 82KD. The expected band size for CD31 is at 82KD.

Figure 3. IHC analysis of CD31 using anti-CD31 antibody (PB9094). CD31 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD31 Antibody (PB9094) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-CD31/PECAM1 Antibody
Boster
catalog: PA1950-1
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Anti-CD31 antibody, PA1950-1, IHC(P). IHC(P): Human Intestinal Cancer Tissue

Anti-CD31 antibody, PA1950-1, IHC(P). IHC(P): Human Placenta Tissue

Anti-CD31 antibody, PA1950-1, IHC(F). IHC(F): Human Placenta Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-CD31 PECAM1 Rabbit Monoclonal Antibody
Boster
catalog: M01513-1
domestic rabbit monoclonal (CEE-16)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry

Immunohistochemical analysis of paraffin-embedded human kindey, using CD31 Antibody(M01513-1). PECAM1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PECAM1 Antibody (M01513-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of CD31 expression in THP-1 cell lysates (M01513-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PECAM1 monoclonal antibody (Catalog # M01513-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PECAM1
quantity: 100 µl
price: 315 USD
to the supplier
Anti-Human CD31 PECAM1 Monoclonal Antibody FITC Conjugated, Flow Validated
Boster
catalog: FC01513-FITC
mouse monoclonal (JC70)
reactivity: human
conjugate: FITC
application: flow cytometry

Monocytes gated PBMCs stained with FITC conjugated anti-human CD31 (clone JC70) (red histogram). Monocytes gated PBMCs stained with FITC conjugated mouse IgG1 isotype control (Blue histogram). The data are generated by BD Accuri C6 Flow Cytometer and analyzed by FlowJo software.
quantity: 100 Tests, 200 Tests
price: 235 USD
to the supplier
Anti-CD31 PECAM1 Rabbit Monoclonal Antibody
Boster
catalog: M01513-2
domestic rabbit monoclonal (DAI-16)
reactivity: human
application: western blot, immunohistochemistry, flow cytometry

Western blot analysis of CD31 expression in THP1 cell lysate (M01513-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PECAM1 monoclonal antibody (Catalog # M01513-2) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PECAM1
quantity: 100 µl
price: 315 USD
to the supplier
Anti-CD31 PECAM1 Antibody
Boster
catalog: A01513-4
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
quantity: 100 ul
price: 697 USD
to the supplier
Anti-CD31/PECAM-1 (T709) Antibody
Boster
catalog: A01513T709
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
quantity: 100 ul
price: 512 USD
to the supplier
Anti-Phospho-CD31 (Y713) PECAM1 Antibody
Boster
catalog: A01513Y713
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
quantity: 100 ul
price: 380 USD
to the supplier
Anti-Human CD31 PECAM1 Monoclonal Antibody Unconjugated Conjugated, Flow Validat ...
Boster
catalog: FC01513
mouse monoclonal (JC70)
reactivity: human
application: flow cytometry
quantity: 100 ug, 500 ug
price: 147 USD
to the supplier
Anti-CD31 (PECAM-1) Rabbit Monoclonal Antibody, Clone#RM247
Boster
catalog: M01513-3
domestic rabbit monoclonal (RM247)
reactivity: human
application: western blot, immunohistochemistry
quantity: 100 ul
price: 399 USD
to the supplier
Anti-CD31/PECAM1 Antibody Picoband™ (monoclonal, 2D4)
Boster
catalog: M01513-4
mouse monoclonal (2D4)
reactivity: human
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
quantity: 100µg/vial
price: 315 USD
to the supplier