domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
reactivity: human, mouse, rat
application: western blot, ELISA
Figure 1. Western blot analysis of IL-22 using anti-IL-22 antibody (A00963-1). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysate,. Lane 2: mouse HEPA1-6 whole cell lysate,. Lane 3: rat PC-12 whole cell lysate, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-22 antigen affinity purified polyclonal antibody (Catalog # A00963-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-22 at approximately 22KD. The expected band size for IL-22 is at 20KD.
quantity: 100 ug/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot
reactivity: human, mouse, rat, zebrafish
application: western blot
Figure 1. Western blot analysis of IL22 using anti-IL22 antibody (A00963-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human placenta tissue lysates,. Lane 3: rat thymus tissue lysates,. Lane 4: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL22 antigen affinity purified polyclonal antibody (Catalog # A00963-2) at 0.5 ug/mL overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL22 at approximately 20KD. The expected band size for IL22 is at 20KD.
quantity: 100 ug/vial
price: 315 USD
to the supplier
mouse monoclonal (7F2)
reactivity: human
application: western blot, flow cytometry
reactivity: human
application: western blot, flow cytometry
Figure 2. Western blot analysis of IL22 using anti- IL22 antibody (M00963). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, . Lane 2: human A375 whole cell lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- IL22 antigen affinity purified monoclonal antibody (Catalog # M00963) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for IL22 at approximately 24KD. The expected band size for IL22 is at 20KD.
quantity: 100µg/vial
price: 315 USD
to the supplier
mouse monoclonal (7F2)
reactivity: human
application: flow cytometry
reactivity: human
application: flow cytometry
mouse monoclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry
reactivity: human
conjugate: DyLight 550
application: flow cytometry
