domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Anti-LOX 1 antibody, PA1832, Western blotting. All lanes: Anti LOX 1 (PA1832) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: SMMC Whole Cell Lysate at 40ug. Lane 3: U87 Whole Cell Lysate at 40ug. Lane 4: U937 Whole Cell Lysate at 40ug. Lane 5: K562 Whole Cell Lysate at 40ug. Predicted bind size:31 KD. Observed bind size: 31KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, rat
application: western blot
reactivity: human, rat
application: western blot
Anti- LOX1 antibody, PA1833, Western blotting. All lanes: Anti LOX1 (PA1833) at 0.5ug/ml. Lane 1: Rat Liver Tissue Lysate at 50ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: K562 Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Lane 5: RAJI Whole Cell Lysate at 40ug. Predicted bind size: 31KD. Observed bind size: 38KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Figure 1. Western blot analysis of LOX-1/OLR1 using anti-LOX-1/OLR1 antibody (A00760-1). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX-1/OLR1 antigen affinity purified polyclonal antibody (Catalog # A00760-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX-1/OLR1 at approximately 38KD. The expected band size for LOX-1/OLR1 is at 31KD.
quantity: 100 ug/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry