Anti-TrkB/NTRK2 Antibody
Boster
catalog: PA2150
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
domestic rabbit monoclonal (EGA-14)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of Phospho-TrkB (Y817) expression in SH-SY5Y cell lysate treated with BDNF (P01388). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NTRK2 monoclonal antibody (Catalog # P01388) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NTRK2

Figure 2. IHC analysis of paraffin-embedded section of mouse brain cancer tissues using anti-Phospho-TrkB (Y817) antibody (P01388). Phospho-TrkB (Y817) was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Phospho-TrkB (Y817) Antibody (P01388) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
quantity: 100 ul
price: 512 USD
to the supplier
Anti-TrkB (Ab-705) NTRK2 Antibody
Boster
catalog: A01388-1
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunocytochemistry
quantity: 100 ul
price: 299 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry
quantity: 100 ul
price: 547 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry
quantity: 100 ul
price: 380 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
quantity: 100 ul
price: 547 USD
to the supplier
Anti-Phospho-Trk B (Y706) NTRK2 Antibody
Boster
catalog: A01388Y706-1
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
quantity: 100 ul
price: 380 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
quantity: 100 ul
price: 697 USD
to the supplier
Anti-NTRK2 Antibody
Boster
catalog: M01388
mouse monoclonal (monoclonal)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
quantity: 80 ul
price: 170 USD
to the supplier
domestic rabbit monoclonal (17N23)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
quantity: 100 µl/vial
price: 315 USD
to the supplier