Anti-TrkA/NTRK1 Antibody
Boster
catalog: PA2149-1

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-TrkA antibody, PA2149-1, Western blotting. Lane 1: COLO320 Cell Lysate. Lane 2: HT1080 Cell Lysate. Lane 3: U87 Cell Lysate.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-TrkA NTRK1 Rabbit Monoclonal Antibody
Boster
catalog: M00706-1

domestic rabbit monoclonal (CDH-14)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of TrkA expression in Human fetal brain tissue lysate (M00706-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NTRK1 monoclonal antibody (Catalog # M00706-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NTRK1

Immunohistochemical analysis of paraffin-embedded human liver, using TrkA Antibody(M00706-1). NTRK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NTRK1 Antibody (M00706-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-TrkA/NTRK1 Antibody Picoband™
Boster
catalog: A00706

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Figure 1. Western blot analysis of TrkA using anti-TrkA antibody (A00706). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human SGC-7901 whole cell lysates,. Lane 3: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TrkA antigen affinity purified polyclonal antibody (Catalog # A00706) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TrkA at approximately 150KD. The expected band size for TrkA is at 87KD.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-TrkA+B+C NTRK1 Rabbit Monoclonal Antibody
Boster
catalog: M00706

domestic rabbit monoclonal (HHH-14)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Western blot analysis of TrkA+B+C expression in Human fetal brain lysate (M00706). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NTRK1 monoclonal antibody (Catalog # M00706) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NTRK1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Phospho-Trk A (Y680/Y681) NTRK1 Antibody
Boster
catalog: A00706Y680Y681

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA

quantity: 100 ul
price: 380 USD
to the supplier

Anti-Phospho-Trk A (Y701) NTRK1 Antibody
Boster
catalog: A00706Y701

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-Phospho-Trk A (Y757) NTRK1 Antibody
Boster
catalog: A00706Y757

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: ELISA, immunohistochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-Phospho-Trk A (Y791) NTRK1 Antibody
Boster
catalog: A00706Y791

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: ELISA, immunohistochemistry

quantity: 100 ul
price: 380 USD
to the supplier