Anti-nNOS (neuronal)/NOS1 Antibody Picoband™
Boster
catalog: A01070
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Western blot analysis of nNOS (neuronal) expression in rat brain extract (lane 1) and mouse brain extract (lane 2). nNOS (neuronal) at 160KD was detected using rabbit anti- nNOS (neuronal) Antigen Affinity purified polyclonal antibody (Catalog #A01070) at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1)
Boster
catalog: MA1072
mouse monoclonal (NOS-B1)
reactivity: human, rat
application: western blot

Boster Kit Box
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-nNOS (neuronal)/NOS1 Antibody
Boster
catalog: PA1329
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Anti-nNOS(neuronal) antibody, PA1329, Western blotting. All lanes: Anti nNOS(neuronal)(PA1329) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: MCF-7 Whole Cell Lysate at 40ug. Predicted bind size: 160KD. Observed bind size: 160KD

Anti-nNOS(neuronal) antibody, PA1329, IHC(P). IHC(P): Rat Brain Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-nNOS (neuronal) NOS1 Rabbit Monoclonal Antibody
Boster
catalog: M01070
domestic rabbit monoclonal (CEI-14)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of nNOS expression in Mouse brain cell lysate (M01070). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOS1 monoclonal antibody (Catalog # M01070) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOS1

Immunohistochemical analysis of paraffin-embedded mouse kidney, using nNOS (neuronal) Antibody(M01070). NOS1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NOS1 Antibody (M01070)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier
Anti-nNOS (neuronal)/NOS1 Antibody Picoband™
Boster
catalog: A01070-2
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, flow cytometry
quantity: 100 µg/vial
price: 315 USD
to the supplier
Anti-NOS1 (phospho-S1417) Antibody
Boster
catalog: A01070S1417
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity: 100 ul
price: 547 USD
to the supplier
Anti-Phospho-NOS1 (S852) Antibody
Boster
catalog: A01070S852
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
quantity: 100 ul
price: 380 USD
to the supplier
Anti-NOS1 (Y846) Antibody
Boster
catalog: A01070Y846
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
quantity: 100 ul
price: 512 USD
to the supplier