mouse monoclonal (90000000000)
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry
domestic rabbit monoclonal (EAC-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-c-Myc (S62) Antibody (P00026-2). MYC was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYC Antibody (P00026-2)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Phospho-c-Myc (S62) expression in HeLa cell lysate (P00026-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYC monoclonal antibody (Catalog # P00026-2) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYC
quantity: 100 µl
price: 315 USD
to the supplier
mouse monoclonal (SPM237)
reactivity: human
application: immunohistochemistry
Anti-c-Myc Antibody Picoband™
Boster
catalog: PB9092
domestic rabbit polyclonal
reactivity: human, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Anti-c-Myc Monoclonal Antibody
Boster
catalog: M00026-1
domestic rabbit monoclonal (AHI-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

Immunofluorescent analysis of HeLa cells, using c-Myc Antibody.

Figure 2. IHC analysis of MYC using anti-MYC antibody (M00026-1)on paraffin-embedded human lung cancer. MYC was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYC Antibody (M00026-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of MYC using anti-MYC antibody (M00026-1) in 1) Jurkat cell lysate;(2) K562 cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYC antigen affinity purified polyclonal antibody (Catalog # M00026-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MYC.
quantity: 100 µl
price: 315 USD
to the supplier
Anti-Myc (R367) Antibody
Boster
catalog: A00026
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: immunohistochemistry
quantity: 100 ul
price: 512 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, flow cytometry
quantity: 100 µg/vial
price: 315 USD
to the supplier
Anti-Phospho-c-Myc (S373) Antibody
Boster
catalog: A00026S373
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry, immunoprecipitation
quantity: 100 ul
price: 380 USD
to the supplier
Anti-c-Myc (Phospho-S62) Antibody
Boster
catalog: A00026S62-1
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
quantity: 100 ul
price: 697 USD
to the supplier
Anti-Phospho-c-Myc (T358) Antibody
Boster
catalog: A00026T358
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry, immunoprecipitation
quantity: 100 ul
price: 380 USD
to the supplier
Anti-c-Myc (Phospho-T58) Antibody
Boster
catalog: A00026T58-1
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
quantity: 100 ul
price: 697 USD
to the supplier
Anti-c-Myc (Phospho-T58/S62) Antibody
Boster
catalog: A00026T58S62
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation
quantity: 100 ul
price: 697 USD
to the supplier
Anti-MYC Antibody
Boster
catalog: M00026-3
mouse monoclonal (monoclonal)
reactivity: human
application: western blot
quantity: 50 ul
price: 170 USD
to the supplier