Anti-Episialin, EMA MUC1 Antibody (Monoclonal, GP1.4)
Boster
catalog: MA1039

mouse monoclonal (GP1.4)
reactivity: human
application: immunohistochemistry

Anti- Episialin, EMA antibody, MA1039, IHC(P). IHC(P): Human Intestinal Cancer Tissue

Anti- Episialin, EMA antibody, MA1039, IHC(P). IHC(P): Human Lung Cancer Tissue

Anti- Episialin, EMA antibody, MA1039, IHC(P). IHC(P): Human Mammary Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Mucin-1 MUC1 Antibody
Boster
catalog: PA2302

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-MUC1 antibody, PA2302, All Western blotting. All lanes: Anti-MUCIN1(PA2302) at 0.5ug/ml. Lane 1: COLO320 Whole Cell Lysate at 40ug. Lane 2: SW620 Whole Cell Lysate at 40ug. Lane 3: HEPG2 Whole Cell Lysate at 40ug. Lane 4: MCF-7 Whole Cell Lysate at 40ug. Lane 5: JURKAT Whole Cell Lysate at 40ug. Predicted bind size: 122KD. Observed bind size: 170KD
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-MUC1 Antibody Picoband™
Boster
catalog: A00187

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Figure 1. Western blot analysis of MUC1 using anti-MUC1 antibody (A00187). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates,. Lane 2: rat pancreas tissue lysates,. Lane 3: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC1 antigen affinity purified polyclonal antibody (Catalog # A00187) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC1 at approximately 122KD. The expected band size for MUC1 is at 122KD.

Figure 2. IHC analysis of MUC1 using anti-MUC1 antibody (A00187). MUC1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MUC1 Antibody (A00187) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of MUC1 using anti-MUC1 antibody (A00187). MUC1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MUC1 Antibody (A00187) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-MUC1 Rabbit Monoclonal Antibody
Boster
catalog: M00187-1

domestic rabbit monoclonal (FHA-13)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of MUC1 expression in T47 D cell lysate (M00187-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC1 monoclonal antibody (Catalog # M00187-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC1

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using MUC1 Antibody(M00187-1) . MUC1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MUC1 Antibody (M00187-1)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin- Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-MUC1 Antibody Picoband™
Boster
catalog: A00187-2

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, flow cytometry, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-MUC1 Antibody Picoband™
Boster
catalog: A00187-3

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: ELISA, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 µg/vial
price: 315 USD
to the supplier