domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
reactivity: human, mouse
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
Figure 1. Western blot analysis of Myeloperoxidase/MPO using anti- Myeloperoxidase/MPO antibody (PA1054). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Myeloperoxidase/MPO antigen affinity purified polyclonal antibody (Catalog # PA1054) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Figure 2. IHC analysis of Myeloperoxidase/MPO using anti- Myeloperoxidase/MPO antibody (PA1054). Myeloperoxidase/MPO was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Myeloperoxidase/MPO Antibody (PA1054) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IF analysis of Myeloperoxidase/MPO using anti- Myeloperoxidase/MPO antibody (PA1054). Myeloperoxidase/MPO was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti- Myeloperoxidase/MPO Antibody (PA1054) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry
reactivity: human, mouse
application: western blot, immunohistochemistry
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunoprecipitation
reactivity: human
application: western blot, immunoprecipitation
domestic rabbit monoclonal (AOCF-13)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry