domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
domestic rabbit monoclonal (CHE-13)
reactivity: human
application: western blot
reactivity: human
application: western blot
Western blot analysis of MMP12 expression in WI-38 cell lysate (M01520). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP12 monoclonal antibody (Catalog # M01520) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP12
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry
reactivity: human
application: western blot, immunohistochemistry
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry
reactivity: human
application: western blot, ELISA, immunohistochemistry
Anti-Matrix metalloproteinase-12 (MMP-12) Rabbit Monoclonal Antibody, Clone#RM38 ...
Boster
catalog: M01520-1
Boster
catalog: M01520-1
domestic rabbit monoclonal (RM381)
reactivity: human
application: western blot, immunohistochemistry
reactivity: human
application: western blot, immunohistochemistry