mouse monoclonal (FR4D11)
reactivity: human
application: flow cytometry
reactivity: human
application: flow cytometry
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
mouse monoclonal (FR4D11)
reactivity: human
conjugate: PE
application: flow cytometry
reactivity: human
conjugate: PE
application: flow cytometry
mouse monoclonal (FR4D11)
reactivity: human
conjugate: FITC
application: flow cytometry
reactivity: human
conjugate: FITC
application: flow cytometry
rabbit monoclonal (GEH-13)
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of CD10 expression in Jurkat lysate (M04065). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MME monoclonal antibody (Catalog # M04065) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MME
quantity: 100 ug/vial
price: 299 USD
to the supplier