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> Boster Ki 67 antibody
Anti-Ki67/MKI67 Antibody
Boster
catalog: PA1918
domestic rabbit polyclonal
reactivity:
human
application:
western blot
Anti-Ki67 antibody, PA1918, Western blotting. Recombinant Protein Detection Source: E.coli derived -recombinant Human Ki67 50.3KD (162aa tag+ K2967-I3256). Lane 1: Recombinant Human Ki67 Protein 10ng . Lane 2: Recombinant Human Ki67 Protein 5ng . Lane 3: Recombinant Human Ki67 Protein 2.5ng
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-Ki67/MKI67 Antibody Picoband™
Boster
catalog: PB9026
domestic rabbit polyclonal
reactivity:
human
application:
western blot
,
immunocytochemistry
,
immunohistochemistry - paraffin section
Anti-Ki67 Picoband antibody, PB9026-1.JPG. IHC(P): Human Tonsil Tissue
Anti-Ki67 Picoband antibody, PB9026-2.jpg. All lanes: Anti-Ki67(PB9026) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: MCF-7 Whole Cell Lysate at 40ug . Lane 3: COLO320 Whole Cell Lysate at 40ug. Lane 4: HEPG2 Whole Cell Lysate at 40ug. Lane 5: SKOV Whole Cell Lysate at 40ug. Predicted bind size: 358KD. Observed bind size: 358KD
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-Ki-67 MKI67 Antibody
Boster
catalog: M00254-4
chicken polyclonal
reactivity:
human
,
mouse
,
rat
application:
western blot
,
immunocytochemistry
Western blot analysis of equal amounts of cell lysates probed with chicken pAb to Ki-67, M00254-4, dilution 1:5,000, in green: [1] protein standard (red), [2] HeLa cells, [3] HEK293 cells. Strong double bands above 250kDa correspond to the two major isoforms of molecular weight 345kDa and 395kDa of Ki-67. Since Ki-67 is a rather unstable multiple protein proteolytic fragments of these isoforms are also detected on the blot.
Immunofluorescent analysis of HeLa cells stained with chicken pAb to Ki-67, M00254-4, dilution 1:2,000 in red, and mouse mAb to ?-tubulin
quantity: 100 ul
price: 299 USD
to the supplier
Anti-Ki67 MKI67 Rabbit Monoclonal Antibody
Boster
catalog: M00254
domestic rabbit monoclonal (EAH-13)
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
,
flow cytometry
Immunohistochemical analysis of paraffin-embedded human tonsil, using Ki67 Antibody (M00254). MKI67 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MKI67 Antibody (M00254)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of Ki67 expression in Ramos cell lysate (M00254). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MKI67 monoclonal antibody (Catalog # M00254) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MKI67
quantity: 100 µl
price: 315 USD
to the supplier
Anti-Human Ki-67 Monoclonal Antibody
Boster
catalog: M00254-1
mouse monoclonal (monoclonal)
reactivity:
human
application:
western blot
,
immunocytochemistry
quantity: 100 ul
price: 299 USD
to the supplier
Anti-Human Ki-67 Monoclonal Antibody
Boster
catalog: M00254-2
mouse monoclonal (monoclonal)
reactivity:
human
application:
western blot
,
immunocytochemistry
quantity: 100 ul
price: 299 USD
to the supplier
Anti-Human Ki67 Antibody
Boster
catalog: M00254-3
domestic rabbit polyclonal (polyclonal)
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
quantity: 100 ul
price: 299 USD
to the supplier
Anti-KI67 MKI67 Rabbit Monoclonal Antibody, Clone#RM360
Boster
catalog: M00254-5
domestic rabbit monoclonal (RM360)
reactivity:
human
application:
western blot
,
immunohistochemistry
quantity: 100 uL
price: 399 USD
to the supplier
Anti-Ki 67 MKI67 Monoclonal Antibody
Boster
catalog: M00254-7
mouse monoclonal (4A8)
reactivity:
human
application:
immunohistochemistry
quantity: 100 ul
price: 380 USD
to the supplier
Anti-Ki67 Antibody Picoband™ (monoclonal, 5E12)
Boster
catalog: M00254-8
mouse monoclonal (5E+12)
reactivity:
human
application:
immunocytochemistry
,
flow cytometry
,
immunohistochemistry - paraffin section
quantity: 100µg/vial
price: 315 USD
to the supplier
Anti-Ki67 Antibody Picoband™ (monoclonal, 5C7)
Boster
catalog: M00254-9
mouse monoclonal (5C7)
reactivity:
human
application:
western blot
,
immunocytochemistry
,
flow cytometry
,
immunohistochemistry - paraffin section
quantity: 100 µg/vial
price: 315 USD
to the supplier