domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
Anti-MIF antibody, PA1052, Western blotting. Lane 1: HELA Cell Lysate. Lane 2: COLO320 Cell Lysate. Lane 3: MM231 Cell Lysate
Figure 2. IHC analysis of MIF using anti-MIF antibody (PA1052). MIF was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MIF Antibody (PA1052) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of MIF using anti-MIF antibody (PA1052). MIF was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MIF Antibody (PA1052) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
Anti- MIF Picoband antibody, PB9274, Western blotting. All lanes: Anti MIF (PB9274) at 0.5ug/ml. Lane 1: U87 Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Lane 3: HUT Whole Cell Lysate at 40ug. Lane 4: A549 Whole Cell Lysate at 40ug. Lane 5: HEPG2 Whole Cell Lysate at 40ug. Predicted bind size: 12KD. Observed bind size: 12KD
Anti- MIF Picoband antibody, PB9274, IHC(P). IHC(P): Human Tonsil Tissue
Anti- MIF Picoband antibody, PB9274, ICC. ICC: JURKAT Cell
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA
Figure. Western blot analysis of MIF using anti- MIF antibody (RP1020). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane : Recombinant Human MIF Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- MIF antigen affinity purified polyclonal antibody (Catalog # RP1020) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIF at approximately 12KD. The expected band size for MIF is at 12KD.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (ABAG-13)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry
reactivity: human
application: western blot, immunoprecipitation, flow cytometry