domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Anti-Midkine antibody, RP1051, Western blotting. All lanes: Anti Midkine (RP1051) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: SMMC Whole Cell Lysate at 40ug. Lane 3: SW620 Whole Cell Lysate at 40ug. Predicted bind size: 16KD. Observed bind size: 38KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (EBI-13)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
Western blot analysis of Midkine expression in Midkine Recombinant protein (M01823). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDK monoclonal antibody (Catalog # M01823) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDK
Immunohistochemical analysis of paraffin-embedded human liver carcinoma, using Midkine Antibody (M01823). MDK was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MDK Antibody (M01823)overnight at 4 C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot
reactivity: human
application: western blot
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry
reactivity: human
application: western blot, ELISA, immunohistochemistry