domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Figure 1. Western blot analysis of Myoglobin using anti-Myoglobin antibody (A04058). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysate,. Lane 2: human Hela whole cell lysate,. Lane 3: human HL-60 whole cell lysate,. Lane 4: human Jurkat whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myoglobin antigen affinity purified polyclonal antibody (Catalog # A04058) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myoglobin at approximately 20KD. The expected band size for Myoglobin is at 17KD.
quantity: 100 ug/vial
price: 315 USD
to the supplier
mouse monoclonal (MG-1)
reactivity: human, rat
application: immunohistochemistry
reactivity: human, rat
application: immunohistochemistry
Anti-Myoglobin antibody (monoclonal), MA1062, Western blotting. WB: Rat Skeletal Muscle Tissue Lysate
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
domestic rabbit monoclonal (FOD-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
Western blot analysis of Myoglobin expression in Human heart muscle lysate (M04058). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MB monoclonal antibody (Catalog # M04058) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MB
quantity: 100 µl
price: 315 USD
to the supplier