mouse monoclonal (HM-2)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
Anti-MAP2 antibody (monoclonal), MA1057, IHC(P). IHC(P): Rat Brain Tissue
quantity: 100 ug/vial
price: 280 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
reactivity: human, mouse, rat
application: western blot, ELISA
Figure 1. Western blot analysis of MAP2 using anti-MAP2 antibody (A01201). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2 antigen affinity purified polyclonal antibody (Catalog # A01201) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2 at approximately 280KD. The expected band size for MAP2 is at 199KD.
quantity: 100 ug/vial
price: 240 USD
to the supplier
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
Figure 1. Western blot analysis of MAP2 using anti-MAP2 antibody (A01201-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2 antigen affinity purified polyclonal antibody (Catalog # A01201-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MAP2.
Immunofluorescence staining of paraffin-embedded Glioma cells of Spinal Cord using MAP2 Antibody #A01201-1.
quantity: 100 ul
price: 299 USD
to the supplier
chicken monoclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
Western blot analysis of whole brain tissue lysates using chicken pAb to microtubule associated protein 2 (MAP2), CPCA-MAP2, dilution 1:50,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. Strong band at ~280kDa mark corresponds to two major isoforms of MAP2 protein referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected if the antibody is used at higher concentrations.
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to microtubule associated protein 2 (MAP2), cpca-map2, dilution 1:10,000 in red, and mouse mAb to MAP-tau, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. cpca-map2 antibody stains dendrites and perikarya of neurons, while M01201-3 antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
quantity: 100 ul
price: 299 USD
to the supplier