Anti-Smad2 Antibody
Boster
catalog: PA1898

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section

Anti-Smad2 antibody, PA1898, Western blotting. Recombinant Protein Detection Source: E.coli derived -recombinant human SHC1, 35.0KD (162aa tag+D424-P578). Lane 1: Recombinant Human SHC1 Proteins 10ng . Lane 2: Recombinant Human SHC1 Proteins 5ng . Lane 3: Recombinant Human SHC1 Proteins 2.5ng

Anti-Smad2 antibody, PA1898, IHC(P). IHC(P): Human Mammary Cancer Tissue

Anti-Smad2 antibody, PA1898, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: HELA Cell Lysate. Lane 3: SMMC Cell Lysate. Lane 4: JURKAT Cell Lysate. Lane 5: U87 Cell Lysate. Lane 6: MCF-7 Cell Lysate.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Phospho-Smad2 (S255) Rabbit Monoclonal Antibody
Boster
catalog: P00090

domestic rabbit monoclonal (HBE-19)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation

Western blot analysis of Phospho-Smad2 (S255) expression in Hela cell treated with Okadaic acid and Calyculin A lysate (P00090). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # P00090) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Smad2 Rabbit Monoclonal Antibody
Boster
catalog: M00090-1

domestic rabbit monoclonal (ACI-19)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry

Immunohistochemical analysis of paraffin-embedded rat stomach, using Smad2 Antibody(M00090-1). SMAD2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD2 Antibody (M00090-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Smad2 expression in (1) Jurkat cell lysate;(2) RAW2647 cell lysate (M00090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # M00090-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Smad2 Rabbit Monoclonal Antibody
Boster
catalog: M00090

domestic rabbit monoclonal (EFF-19)
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of Smad2 expression in Jurkat cell lysate (M00090). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # M00090) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2
quantity: 100 µl
price: 315 USD
to the supplier

Anti-SMAD2 Antibody
Boster
catalog: A00090-1

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of SMAD2 using anti-SMAD2 antibody (A00090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. Lane 2: rat liver tissue lysates. Lane 3: mouse testicular tissue lysates. Lane 4: mouse heart tissue lysates. Lane 5: mouse lung tissue lysates. Lane 6: mouse lung tissue lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 antigen affinity purified polyclonal antibody (Catalog # A00090-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2 at approximately 60KD. The expected band size for SMAD2 is at 52KD.

Figure 2. IHC analysis of SMAD2 using anti-SMAD2 antibody (A00090-1). SMAD2 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD2 Antibody (A00090-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of SMAD2 using anti-SMAD2 antibody (A00090-1). SMAD2 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD2 Antibody (A00090-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Phospho-Smad2 (S250) Rabbit Monoclonal Antibody
Boster
catalog: P00090-1

domestic rabbit monoclonal (HBC-19)
reactivity: human, mouse, rat
application: western blot, flow cytometry

Western blot analysis of Phospho-Smad2 (S250) expression in (1) HeLa cell treated with alkaline phosphatase lysate; (2) HeLa cell lysate (P00090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # P00090-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2
quantity: 100 µl
price: 315 USD
to the supplier

Anti-SMAD2 Antibody Picoband™ (monoclonal, 3C4)
Boster
catalog: M00090-3

mouse monoclonal (3C4)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier