Anti-SMAD1/SMAD2/SMAD3/SMAD5 Antibody
Boster
catalog: PA1331

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Anti-SMAD 1/2/3/5 antibody, PA1331, Western blotting. Lane 1: Rat Heart Tissue Lysate. Lane 2: Rat Skeletal Muscle Tissue Lysate. Lane 3: Rat Kidney Tissue Lysate. Lane 4: Rat Brain Tissue Lysate. Lane 5: MM453 Cell Lysate. Lane 6: MM231 Cell Lysate . Lane 7: HELA Cell Lysate. Lane 8: SMMC Cell Lysate. Lane 9: SW620 Cell Lysate
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Smad1 Antibody
Boster
catalog: PA2114

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Anti-Smad1 antibody, PA2114, Western blotting. Lane 1: SMMC Cell Lysate. Lane 2: K562 Cell Lysate. Lane 3: HT1080 Cell Lysate. Lane 4: HELA Cell Lysate. Lane 5: JURKAT Cell Lysate.

Anti-Smad1 antibody, PA2114, IHC(P). IHC(P): Human Lung Cancer Tissue

Anti-Smad1 antibody, PA2114, ICC. ICC: A549 Cell
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-SMAD1 Antibody Picoband™
Boster
catalog: PB9395

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Anti- SMAD1 Picoband antibody, PB9395, Western blotting. All lanes: Anti SMAD1 (PB9395) at 0.5ug/ml. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug. Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug. Lane 3: Rat Skeletal Muscle Tissue Lysate at 50ug. Lane 4: Mouse Skeletal Muscle Tissue Lysate at 50ug. Lane 5: 293T Whole Cell Lysate at 40ug. Lane 6: MCF-7 Whole Cell Lysate at 40ug. Lane 7: HELA Whole Cell Lysate at 40ug. Predicted bind size: 52KD. Observed bind size: 52KD
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-SMAD1/SMAD5 Antibody Picoband™
Boster
catalog: A00728-1

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Figure 1. Western blot analysis of SMAD1 using anti-SMAD1 antibody (A00728-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate,. Lane 2: rat testis tissue lysate,. Lane 3: mouse brain tissue lysate,. Lane 4: mouse kidney tissue lysate,. Lane 5: mouse testis tissue lysate,. Lane 6: human Hela cell lysate,. Lane 7: human A375 cell lysate,. Lane 8: human HepG2 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD1 antigen affinity purified polyclonal antibody (Catalog # A00728-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD1 at approximately 52,60KD. The expected band size for SMAD1 is at 48, 52, 60KD.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Smad1 Rabbit Monoclonal Antibody
Boster
catalog: M00728-1

domestic rabbit monoclonal (CAC-19)
reactivity: human, mouse
application: western blot, immunocytochemistry

Immunohistochemical analysis of paraffin-embedded human kidney, using Smad1 Antibody(M00728-1). SMAD1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD1 Antibody (M00728-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of Smad1 expression in HeLa cell lysate (M00728-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD1 monoclonal antibody (Catalog # M00728-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Smad1 Rabbit Monoclonal Antibody
Boster
catalog: M00728

domestic rabbit monoclonal (HCH-19)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Western blot analysis of Smad1 expression in 293T cell lysate (M00728). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD1 monoclonal antibody (Catalog # M00728) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD1
quantity: 100 µl
price: 315 USD
to the supplier