domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
Figure 1. IHC analysis of Galectin-3 using anti-Galectin-3 antibody (PB9081). Galectin-3 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Galectin-3 Antibody (PB9081) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of Galectin-3 using anti-Galectin-3 antibody (PB9081). Galectin-3 was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Galectin-3 Antibody (PB9081) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. Western blot analysis of Galectin-3 using anti-Galectin-3 antibody (PB9081). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Hela Whole Cell Lysate. Lane 2: SGC Whole Cell Lysate. Lane 3: MCF-7 Whole Cell Lysate. Lane 4: SW620 Whole Cell Lysate. Lane 5: A375 Whole Cell Lysate. Lane 6: Smmc Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin-3 antigen affinity purified polyclonal antibody (Catalog # PB9081) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin-3 at approximately 26KD. The expected band size for Galectin-3 is at 26KD.
quantity: 100μg/vial
price: 315 USD
to the supplier
mouse monoclonal (12B12)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
domestic rabbit monoclonal (AOIE-12)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
reactivity: human
application: western blot, immunocytochemistry, flow cytometry
mouse monoclonal (monoclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry