Anti-CD18/ITGB2 Antibody
Boster
catalog: PA1124

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-CD18 antibody, PA1124, IHC(P). IHC(P): Human Uroepithelium Cancer Tissue

Anti-CD18 antibody, PA1124, Western blotting. All lanes: Anti CD18 (PA1124) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Lane 3: HT1080 Whole Cell Lysate at 40ug. Predicted bind size: 85KD. Observed bind size: 85KD
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CD18/ITGB2 Antibody
Boster
catalog: PA1623

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Anti-CD18 antibody, PA1623, Western blotting. Lane 1: JURKAT Cell Lysate . Lane 2: CEM Cell Lysate. Lane 3: HT1080 Cell Lysate. Lane 4: SMMC Cell Lysate. Lane 5: HELA Cell Lysate.

Anti-CD18 antibody, PA1623, IHC(P). IHC(P): Human Mammary Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CD18/ITGB2 Antibody Picoband™
Boster
catalog: PB9141

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of CD18 using anti-CD18 antibody (PB9141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human CD18 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD18 antigen affinity purified polyclonal antibody (Catalog # PB9141) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD18 at approximately 40KD. The expected band size for CD18 is at 40KD.

Figure 2. Western blot analysis of CD18 using anti-CD18 antibody (PB9141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: JURKAT Whole Cell Lysate. Lane 2: CEM Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD18 antigen affinity purified polyclonal antibody (Catalog # PB9141) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD18 at approximately 100KD. The expected band size for CD18 is at 85KD.

Figure 3. IHC analysis of CD18 using anti-CD18 antibody (PB9141). CD18 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD18 Antibody (PB9141) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-CD18 ITGB2 Antibody Picoband™ (monoclonal, 1A3/2A10)
Boster
catalog: M00458-1

mouse monoclonal (1A3/2A10)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Figure 1. IHC analysis of CD18 using anti-CD18 antibody (M00458-1). CD18 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-CD18 Antibody (M00458-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 2. IHC analysis of CD18 using anti-CD18 antibody (M00458-1). CD18 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-CD18 Antibody (M00458-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 3. Western blot analysis of CD18 using anti-CD18 antibody (M00458-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD18 antigen affinity purified monoclonal antibody (Catalog # M00458-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Human CD18 DyLight® 550 conjugated ITGB2 Antibody(monoclonal, 1A3/2A10)
Boster
catalog: M00458-Dyl550

mouse monoclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier

Anti-CD18 ITGB2 Rabbit Monoclonal Antibody
Boster
catalog: M00458

domestic rabbit monoclonal (AAIH-9)
reactivity: human
application: western blot, immunoprecipitation

quantity: 100 µl
price: 315 USD
to the supplier

Anti-Hu CD18 Purified ITGB2 Monoclonal Antibody
Boster
catalog: M00458-2

mouse monoclonal (MEM-48)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation, flow cytometry

quantity: 0.1 mg (C100)
price: 280 USD
to the supplier