Anti-CD11b/ITGAM Antibody Picoband™
Boster
catalog: PB9140
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Anti-CD11b Picoband antibody , PB9140-1.jpg. All lanes: Anti CD11B (PB9140) at 0.5ug/ml. WB: Recombinant Human CD11B Protein 0.5ng. Predicted bind size: 45KD. Observed bind size: 45KD

Anti-CD11b Picoband antibody , PB9140-2.JPG. IHC(P): Human Tonsil Tissue

Anti-CD11b Picoband antibody , PB9140-3.JPG. IHC(P): Mouse Spleen Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-CD11b/ITGAM Antibody Picoband™
Boster
catalog: A00144-1
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

Figure 1. Western blot analysis of CD11b using anti-CD11b antibody (A00144-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates,. Lane 2: rat thymus tissue lysates,. Lane 3: mouse spleen tissue lysates,. Lane 4: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11b antigen affinity purified polyclonal antibody (Catalog # A00144-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11b at approximately 170KD. The expected band size for CD11b is at 127KD.
quantity: 100µg/vial
price: 315 USD
to the supplier
Anti-CD11b/ITGAM Antibody
Boster
catalog: PA1943-1
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Anti-CD11b antibody, PA1943-1, All Western blotting. All lanes: Anti-ITGAM(PA1943-1) at 0.5ug/ml. Lane 1: JURKAT Whole Cell Lysate at 40ug. Lane 2: RAJI Whole Cell Lysate at 40ug . Predicted bind size: 127KD . Observed bind size: 170KD
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-human CD11b Itgam Monoclonal Antibody APC Conjugated, Flow Validated
Boster
catalog: FC00144-APC
mouse monoclonal (OKM1)
reactivity: human, mouse
conjugate: APC
application: flow cytometry

Monocytes gated PBMCs stained with APC conjugated anti-human CD11b (clone OKM1, red histogram). PBMCs lymphocytes stained with IgG2b APC isotype control (blue histogram).
quantity: 100 Tests, 200 Tests
price: 191 USD
to the supplier
Anti-CD11b ITGAM Rabbit Monoclonal Antibody
Boster
catalog: M00144-1
domestic rabbit monoclonal (GC-9)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry

Immunohistochemical analysis of paraffin-embedded human spleen, using CD11b Antibody(M00144-1). ITGAM was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ITGAM Antibody (M00144-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of CD11b expression in RAW2647 cell lysate (M00144-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGAM monoclonal antibody (Catalog # M00144-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGAM
quantity: 100 µl
price: 315 USD
to the supplier
Anti-CD11b ITGAM Rabbit Monoclonal Antibody
Boster
catalog: M00144
domestic rabbit monoclonal (BCG-9)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of CD11b expression in TF1 cell lysate (M00144). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGAM monoclonal antibody (Catalog # M00144) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGAM

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CD11b Antibody(M00144). ITGAM was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ITGAM Antibody (M00144)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier
Anti-human CD11b Itgam Monoclonal Antibody PerCP Conjugated, Flow Validated
Boster
catalog: FC00144-PerCP
mouse monoclonal (OKM1)
reactivity: human, mouse
conjugate: PerCP
application: flow cytometry

Monocytes gated PBMCs stained with PerCP conjugated anti-human CD11b (clone OKM1, red histogram). PBMCs lymphocytes stained with IgG2b PerCP isotype control (blue histogram).
quantity: 100 Tests, 200 Tests
price: 338 USD
to the supplier
Anti-Human CD11b DyLight® 488 conjugated ITGAM Antibody
Boster
catalog: A00144-Dyl488
domestic rabbit polyclonal
reactivity: human
conjugate: DyLight488
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier
Anti-Human CD11b DyLight® 550 conjugated ITGAM Antibody
Boster
catalog: A00144-Dyl550
domestic rabbit polyclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier
Anti-Human CD11b ITGAM Monoclonal Antibody iFluor647 Conjugated, Flow Validated
Boster
catalog: FC00144-iFluor647
mouse monoclonal (OKM1)
reactivity: human
application: flow cytometry
quantity: 100 Tests, 200 Tests
price: 292 USD
to the supplier
Anti-Human CD11b ITGAM Monoclonal Antibody mFluor450 Conjugated, Flow Validated
Boster
catalog: FC00144-mFluor450
mouse monoclonal (OKM1)
reactivity: human
application: flow cytometry
quantity: 100 Tests
price: 292 USD
to the supplier
Anti-CD11b (integrin alpha-M) ITGAM Rabbit Monoclonal Antibody, Clone#RM290
Boster
catalog: M00144-2
domestic rabbit monoclonal (RM290)
reactivity: human
application: western blot, immunohistochemistry
quantity: 100 uL
price: 399 USD
to the supplier
Anti-CD11B (L1) monoclonal ITGAM Antibody
Boster
catalog: M00144-3
mouse monoclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 ul
price: 547 USD
to the supplier