Anti-IL8/CXCL8 Antibody
Boster
catalog: PA1353

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Anti-IL-8 antibody, PA1353, Western blotting. Lane 1: Recombinant Human IL-18 Protein 10ng. Lane 2: Recombinant Human IL-18 Protein 5ng. Lane 3: Recombinant Human IL-18 Protein 2.5ng

Anti-IL-8 antibody, PA1353, IHC(P). IHC(P): Human Appendicitis Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-IL8/CXCL8 Antibody Picoband™
Boster
catalog: PB9621

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of IL-8 using anti-IL-8 antibody (PB9621). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-8 antigen affinity purified polyclonal antibody (Catalog # PB9621) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-8 at approximately 34KD. The expected band size for IL-8 is at 11KD.

Figure 2. IHC analysis of IL-8 using anti-IL-8 antibody (PB9621). IL-8 was detected in paraffin-embedded section of human appendicitis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IL-8 Antibody (PB9621) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-IL-8 CXCL8 Antibody
Boster
catalog: A00423

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunoprecipitation

quantity: 1 mL
price: 1274 USD
to the supplier

Anti-IL-8 CXCL8 Monoclonal Antibody
Boster
catalog: A00423-1

mouse monoclonal (8B1)
reactivity: human, mouse
application: immunohistochemistry, immunohistochemistry - paraffin section
citations: 1

quantity: 100µl
price: 399 USD
to the supplier

Anti-IL-8 CXCL8 Monoclonal Antibody
Boster
catalog: M00423

mouse monoclonal (3E4D8)
reactivity: human
application: western blot, ELISA

quantity: 100 ul
price: 380 USD
to the supplier

Anti-IL8 Rabbit Monoclonal Antibody
Boster
catalog: M00423-1

domestic rabbit monoclonal (26C79)
reactivity: human
application: western blot

quantity: 100 µl/vial
price: 315 USD
to the supplier