domestic rabbit polyclonal
reactivity: human, rat
application: western blot, flow cytometry
reactivity: human, rat
application: western blot, flow cytometry
Figure 1. Western blot analysis of IL7R alpha using anti-IL7R alpha antibody (PB9948). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL7R alpha antigen affinity purified polyclonal antibody (Catalog # PB9948) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL7R alpha at approximately 70KD. The expected band size for IL7R alpha is at 70KD.
Figure 2. Flow Cytometry analysis of U20S cells using anti- IL7R alpha antibody (PB9948). Overlay histogram showing U20S cells stained with PB9948 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL7R alpha Antibody (PB9948,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 3. Flow Cytometry analysis of U87 cells using anti- IL7R alpha antibody (PB9948). Overlay histogram showing U87 cells stained with PB9948 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL7R alpha Antibody (PB9948,1ug/1x10 6 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
domestic rabbit monoclonal (22I71)
reactivity: human
application: western blot, flow cytometry
reactivity: human
application: western blot, flow cytometry
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot
reactivity: human
application: western blot
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunocytochemistry
reactivity: human
application: western blot, immunocytochemistry