domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Figure. Western blot analysis of IL-6 using anti- IL-6 antibody (RP1012). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane : Recombinant Human IL-6 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- IL-6 antigen affinity purified polyclonal antibody (Catalog # RP1012) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-6 at approximately 20KD. The expected band size for IL-6 is at 20KD.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunoprecipitation
reactivity: human
application: western blot, immunoprecipitation
mouse monoclonal (monoclonal)
reactivity: human
application: western blot, flow cytometry
reactivity: human
application: western blot, flow cytometry
mouse monoclonal (7D12)
reactivity: human
application: ELISA
reactivity: human
application: ELISA