Anti-Interleukin-4 IL4 Antibody
Boster
catalog: PA1424

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-IL-4 antibody, PA1424, Western blotting. Lane 1: Recombinant Human IL-4 Protein 10ng. Lane 2: Recombinant Human IL-4 Protein 5ng. Lane 3: Recombinant Human IL-4 Protein 2.5ng
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Interleukin-4 IL4 Antibody
Boster
catalog: PA1749

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Anti-IL-4 antibody, PA1749, Western blotting. All lanes: Anti IL-4 (PA1749) at 0.5ug/ml. Lane 1: Recombinant Human IL-4 Protein 10ng. Lane 2: Recombinant Human IL-4 Protein 5ng. Lane 3: Recombinant Human IL-4 Protein 2.5ng. Predicted bind size: 14KD. Observed bind size: 14KD

Anti-IL-4 antibody, PA1749, IHC(P). IHC(P): Human Tonsil Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Interleukin-4 IL4 Antibody
Boster
catalog: RP1009

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Figure. Western blot analysis of IL-4 using anti- IL-4 antibody (RP1009). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane: Recombinant Human IL-4 Protein 0.5ng,. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- IL-4 antigen affinity purified polyclonal antibody (Catalog # RP1009) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-4 at approximately 14KD. The expected band size for IL-4 is at 14KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-IL4 Rabbit Monoclonal Antibody
Boster
catalog: M00230

domestic rabbit monoclonal (AADD-9)
reactivity: human
application: western blot, immunocytochemistry

Western blot analysis of IL4 expression in human tonsil lysate (M00230). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL4 monoclonal antibody (Catalog # M00230) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL4
quantity: 100 µl
price: 315 USD
to the supplier

Anti-IL4 Antibody Picoband™
Boster
catalog: A00230

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA

Figure 1. Western blot analysis of IL4 using anti-IL4 antibody (A00230). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human CCRF-CEM whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL4 antigen affinity purified polyclonal antibody (Catalog # A00230) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL4 at approximately 20KD. The expected band size for IL4 is at 17KD.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-IL-4 Antibody
Boster
catalog: A00230-2

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot

quantity: 100 ul
price: 512 USD
to the supplier