domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
Anti-Mannose 6 Phosphate Receptor(Cation independent) antibody, PA2182, IHC(P). IHC(P): Human Intestinal Cancer Tissue
Anti-Mannose 6 Phosphate Receptor(Cation independent) antibody, PA2182, ICC. ICC: HCT116 Cell
Anti-Mannose 6 Phosphate Receptor(Cation independent) antibody, Western blotting. All lanes: Anti Mannose 6 Phosphate Receptor (Cation independent) (PA2182) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Lane 3: 22RV1 Whole Cell Lysate at 40ug. Lane 4: 293T Whole Cell Lysate at 40ug. Predicted bind size: 274KD. Observed bind size: 274KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (DIC-9)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
Immunohistochemical analysis of paraffin-embedded human colon, using M6PR Antibody(M00951). IGF2R was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IGF2R Antibody (M00951)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of extracts of M6PR expression in Jurkat cell lysate (M00951). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF2R monoclonal antibody (Catalog # M00951) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGF2R
quantity: 100 µl
price: 315 USD
to the supplier
Anti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody Picobandâ„ ...
Boster
catalog: A00951
Boster
catalog: A00951
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
Figure 1. Western blot analysis of IGF2R using anti-IGF2R antibody (A00951). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, . Lane 2: human U-87MG whole cell lysates, . Lane 3: human HepG2 whole cell lysates, . Lane 4: human 22RV1 whole cell lysates, . Lane 5: rat brain tissue lysates,. Lane 6: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF2R antigen affinity purified polyclonal antibody (Catalog # A00951) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGF2R at approximately 300KD. The expected band size for IGF2R is at 274KD.
Figure 2. IHC analysis of IGF2R using anti-IGF2R antibody (A00951). IGF2R was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IGF2R Antibody (A00951) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of IGF2R using anti-IGF2R antibody (A00951). IGF2R was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IGF2R Antibody (A00951) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier
mouse monoclonal (4i11)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
mouse monoclonal (6G2)
reactivity: human
application: western blot, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunohistochemistry - paraffin section