Anti-Interferon gamma/IFNG Antibody
Boster
catalog: PA1493

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-IFN gamma antibody, PA1493, Western blotting. All lanes: Anti IFN gamma (PA1493) at 0.5ug/ml. Lane 1: Recombinant Human IFN gamma Protein 10ng. Lane 2: Recombinant Human IFN gamma Protein 5ng. Lane 3: Recombinant Human IFN gamma Protein 2.5ng. Lane 4: Recombinant Human IFN gamma Protein 1.25ng. Predicted bind size: 19KD. Observed bind size: 19KD
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-IFN gamma Antibody
Boster
catalog: RP1002

domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Figure . Western blot analysis of IFN gamma using anti- IFN gamma antibody (RP1002). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane: Recombinant Human IFN gamma Protein 0.5ng . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- IFN gamma antigen affinity purified polyclonal antibody (Catalog # RP1002) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFN gamma at approximately 17KD. The expected band size for IFN gamma is at 17KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Interferon gamma IFNG Rabbit Monoclonal Antibody
Boster
catalog: M00393-1

domestic rabbit monoclonal (AOFD-9)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry

Western blot analysis of Interferon gamma expression in Jurkat cell lysate (M00393-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG monoclonal antibody (Catalog # M00393-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNG
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Interferon gamma/IFNG Antibody Picoband™
Boster
catalog: A00393-3

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of IFNG using anti-IFNG antibody (A00393-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,. Lane 2: human placenta tissue lysates,. Lane 3: human U-87MG whole cell lysates,. Lane 4: human HL-60 whole cell lysates,. Lane 5: human U-937 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG antigen affinity purified polyclonal antibody (Catalog # A00393-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNG at approximately 19KD. The expected band size for IFNG is at 19KD.

Figure 2. Western blot analysis of IFNG using anti-IFNG antibody (A00393-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: rat lung tissue lysates,. Lane 3: mouse brain tissue lysates,. Lane 4: mouse ovary tissue lysates,. Lane 5: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG antigen affinity purified polyclonal antibody (Catalog # A00393-3) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNG at approximately 19KD. The expected band size for IFNG is at 19KD.

Figure 3. IHC analysis of IFNG using anti-IFNG antibody (A00393-3). IFNG was detected in paraffin-embedded section of human sarcoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IFNG Antibody (A00393-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-IFN-Gamma (Q69) IFNG Antibody
Boster
catalog: A00393

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunoprecipitation

quantity: 100 ul
price: 512 USD
to the supplier

Anti-Interferon gamma (IFNG) Monoclonal Antibody
Boster
catalog: M00393

mouse monoclonal (IFNG/466)
reactivity: human
application: immunohistochemistry, immunocytochemistry

quantity: 100 ug/vial
price: 440 USD
to the supplier

Anti- IFN Gamma Monoclonal Antibody
Boster
catalog: M00393-4

mouse monoclonal (AFIG-9)
reactivity: human
application: western blot

quantity: 100µg/vial
price: 299 USD
to the supplier