Anti-ICAM1 Antibody Picoband™
Boster
catalog: PB9004
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry

Anti-ICAM1 Picoband antibody, IHC(P). IHC(P): Human Intestinal Cancer Tissue

Anti-ICAM1 Picoband antibody,IHC(P) . IHC(P): Human Lung Cancer Tissue

Anti-ICAM1 Picoband antibody, Western blotting. All lanes: Anti-ICAM1(PB9004) at 0.5ug/ml. Lane 1: HEPG2 Whole Cell Lysate at 40ug. Lane 2: K562 Whole Cell Lysate at 40ug. Lane 3: A549 Whole Cell Lysate at 40ug. Lane 4: HT1080 Whole Cell Lysate at 40ug. Predicted bind size: 58KD. Observed bind size: 100KD
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-ICAM1 Antibody Picoband™
Boster
catalog: PB9017
domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Anti-ICAM1 Picoband antibody, PB9017-1.JPG. IHC(P): Human Intestinal Cancer Tissue

Anti-ICAM1 Picoband antibody, PB9017-2.jpg. All lanes: Anti-ICAM1(PB9017) at 0.5ug/ml. Lane 1: HEPG2 Whole Cell Lysate at 40ug. Lane 2: K562 Whole Cell Lysate at 40ug. Lane 3: A549 Whole Cell Lysate at 40ug. Lane 4: HT1080 Whole Cell Lysate at 40ug. Predicted bind size: 58KD. Observed bind size: 100KD

Figure 3. IF analysis of ICAM1 using anti-ICAM1 antibody (PB9017) . ICAM1 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti-ICAM1 Antibody (PB9017) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-ICAM1 Antibody Picoband™
Boster
catalog: A00171
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of ICAM1 using anti-ICAM1 antibody (A00171). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysate,. Lane 2: rat thymus tissue lysate,. Lane 3: rat RH35 cell lysate,. Lane 4: mouse spleen tissue lysate,. Lane 5: mouse thymus tissue lysate,. Lane 6: mouse HEPA1-6 cell lysate,. Lane 7: mouse heart tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (Catalog # A00171) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 90KD. The expected band size for ICAM1 is at 58KD.
quantity: 100µg/vial
price: 315 USD
to the supplier
Anti-Human CD54 Icam1 Monoclonal Antibody FITC Conjugated, Flow Validated
Boster
catalog: FC00171-FITC-1
mouse monoclonal (LB-2)
reactivity: human
conjugate: FITC
application: flow cytometry

Daudi gated cells stained with FITC conjugated mouse anti-human CD54 (clone LB-2) (red histogram). Daudi gated cells stained with FITC conjugated mouse IgG2b isotype control (Blue histogram).
quantity: 100 Tests, 200 Tests
price: 235 USD
to the supplier
Anti-ICAM1/Cd54 Rabbit Monoclonal Antibody
Boster
catalog: M00171-1
domestic rabbit monoclonal (DOD-9)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Western blot analysis of ICAM1 expression in Raji cell lysate (M00171-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 monoclonal antibody (Catalog # M00171-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1
quantity: 100 µl
price: 315 USD
to the supplier
Anti-ICAM1 Antibody Picoband™
Boster
catalog: A00171-2
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, flow cytometry
quantity: 100 µg/vial
price: 315 USD
to the supplier
Anti-Phospho-ICAM-1 (Y512) Antibody
Boster
catalog: A00171Y512-1
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA
quantity: 100 ul
price: 380 USD
to the supplier
Anti-CD54 / ICAM-1 Monoclonal Antibody
Boster
catalog: M00171
mouse monoclonal (W-CAM-1; same as Wehi-CAM-1 or 1H4)
reactivity: human
application: immunohistochemistry
quantity: 100 ug/vial
price: 440 USD
to the supplier
Anti-Hu CD54 Purified ICAM1 Monoclonal Antibody
Boster
catalog: M00171-2
mouse monoclonal (MEM-111)
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry
quantity: 0.1 mg (C100)
price: 280 USD
to the supplier
Anti-ICAM1 Antibody Picoband™ (monoclonal, 6F2C3)
Boster
catalog: M00171-3
mouse monoclonal (6F2C3)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 µg/vial
price: 315 USD
to the supplier