mouse monoclonal (LK1)
reactivity: human, rat, chicken
application: western blot, immunohistochemistry - paraffin section
reactivity: human, rat, chicken
application: western blot, immunohistochemistry - paraffin section
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
Anti-HSP60 antibody, PA1106, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: Rat Liver Tissue Lysate. Lane 3: CEM Cell Lysate. Lane 4: HELA Cell Lysate. Lane 5: SMMC Cell Lysate. Lane 6: COLO320 Cell Lysate.
Anti-HSP60 antibody, PA1106, IHC(P). IHC(P): Rat Intestine Tissue
Anti-HSP60 antibody, PA1106, ICC. ICC: SMMC Cell
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
Anti- Hsp60 Picoband antibody, PB9337, Western blotting. All lanes: Anti Hsp60 (PB9337) at 0.5ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50ug. Lane 2: Mouse Kidney Tissue Lysate at 50ug. Lane 3: HEPA Whole Cell Lysate at 40ug. Lane 4: NRK Whole Cell Lysate at 40ug. Lane 5: PC-12 Whole Cell Lysate at 40ug. Lane 6: SW620 Whole Cell Lysate at 40ug. Lane 7: A549 Whole Cell Lysate at 40ug. Lane 8: A431 Whole Cell Lysate at 40ug. Lane 9: HELA Whole Cell Lysate at 40ug. Predicted bind size: 60KD. Observed bind size: 60KD
Anti- Hsp60 Picoband antibody, PB9337, IHC(P). IHC(P): Mouse Intestine Tissue
Anti- Hsp60 Picoband antibody, PB9337, IHC(P). IHC(P): Rat Intestine Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier
mouse monoclonal (6G2)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
domestic rabbit monoclonal (DOI-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
Western blot analysis of Hsp60 expression in HeLa cell lysate (M01280-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPD1 monoclonal antibody (Catalog # M01280-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPD1
quantity: 100 µl
price: 315 USD
to the supplier