Boster HSP90 antibody

Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband™
Boster
catalog: PB9089

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Anti-Hsp90-alpha-Picoband antibody, PB9089-1.JPG. IHC(P): Human Intestinal Cancer Tissue

Anti-Hsp90-alpha-Picoband antibody, PB9089-2.JPG. IHC(P): Mouse Brain Tissue

Anti-Hsp90-alpha-Picoband antibody, PB9089-3.JPG. IHC(P): Rat Brain Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Hsp90 alpha/HSP90AA1 Antibody Picoband™
Boster
catalog: PB9635

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

Anti- Hsp90 alpha Picoband antibody, PB9635, Western blotting. All lanes: Anti Hsp90 alpha (PB9635) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug. Lane 3: Rat Liver Tissue Lysate at 50ug. Lane 4: HELA Whole Cell Lysate at 40ug. Lane 5: JURKAT Whole Cell Lysate at 40ug. Lane 6: 22RV1 Whole Cell Lysate at 40ug. Predicted bind size: 90KD. Observed bind size: 90KD

Anti- Hsp90 alpha Picoband antibody, PB9635, IHC(P). IHC(P): Mouse Testis Tissue

Anti- Hsp90 alpha Picoband antibody, PB9635, IHC(P). IHC(P): Rat Testis Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Hsp90 alpha + beta HSP90AA1 Rabbit Monoclonal Antibody
Boster
catalog: M01103-2

domestic rabbit monoclonal (EBD-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of Hsp90 alpha + beta expression in HEK-293 cell lysate (M01103-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90AA1 monoclonal antibody (Catalog # M01103-2) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP90AA1

Immunohistochemical analysis of paraffin-embedded human liver carcinoma, using Hsp90 alpha + beta Antibody (M01103-2). HSP90AA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSP90AA1 Antibody (M01103-2)overnight at 4â„ƒ. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37â„ƒ. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody
Boster
catalog: M01103-1

domestic rabbit monoclonal (CCF-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Hsp90 alpha antibody(M01103-1). HSP90AA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSP90AA1 Antibody (M01103-1)overnight at 4â„ƒ. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37â„ƒ. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis on HeLa cell lysate using HSP90A Antibody (M01103-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90AA1 monoclonal antibody (Catalog # M01103-1) overnight at 4â„ƒ, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP90AA1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody
Boster
catalog: M01103-3

domestic rabbit monoclonal (BAC-8)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation

Western blot analysis of Hsp90 alpha expression in (1)PC-12 cell lysate; (2)HeLa cell lysate (M01103-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90AA1 monoclonal antibody (Catalog # M01103-3) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP90AA1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Hsp90 alpha HSP90AA1 Rabbit Monoclonal Antibody
Boster
catalog: M01103

domestic rabbit monoclonal (BAD-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Western blot analysis of Hsp90 alpha expression in Jurkat cell lysate (M01103). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90AA1 monoclonal antibody (Catalog # M01103) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP90AA1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Hsp90 alpha Antibody Picoband™ (monoclonal, 6B5)
Boster
catalog: M01103-4

mouse monoclonal (6B5)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier