Anti-Survivin/BIRC5 Antibody Picoband™
Boster
catalog: A00379

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry - paraffin section

Figure 1. Western blot analysis of Survivin using anti- Survivin antibody (A00379). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # A00379) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16KD. The expected band size for Survivin is at 16KD.

Figure 2. IHC analysis of Survivin using anti-Survivin antibody (A00379). Survivin was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Survivin Antibody (A00379) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of Survivin using anti-Survivin antibody (A00379). Survivin was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Survivin Antibody (A00379) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-survivin/BIRC5 Antibody
Boster
catalog: PA1474

domestic rabbit polyclonal
reactivity: human
application: western blot

Anti-survivin antibody, PA1474, Western blotting. Lane 1: HELA Cell Lysate. Lane 2: JURKAT Cell Lysate. Lane 3: CEM Cell Lysate. Lane 4: COLO320 Cell Lysate.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-survivin/BIRC5 Antibody
Boster
catalog: RP1026

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry

Figure 1. Western blot analysis of Survivin using anti-Survivin antibody (RP1026). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse SP2/0 whole cell lysates, . Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # RP1026) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16KD. The expected band size for Survivin is at 16KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Survivin BIRC5 Rabbit Monoclonal Antibody
Boster
catalog: M00379-1

domestic rabbit monoclonal (ABB-2)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of Survivin in Jurkat cell lysate (M00379-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIRC5 monoclonal antibody (Catalog # M00379-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BIRC5

Immunohistochemical analysis of paraffin-embedded human gastric carcinoma, using Survivin Antibody(M00379-1). BIRC5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BIRC5 Antibody (M00379-1)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Survivin BIRC5 Antibody
Boster
catalog: A00379-1

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

quantity: 0.1 mg
price: 404 USD
to the supplier

Anti-Survivin BIRC5 Antibody
Boster
catalog: A00379-2

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 100 ul
price: 697 USD
to the supplier

Anti-Survivin BIRC5 Antibody
Boster
catalog: A00379-3

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-Phospho-Survivin (T117) BIRC5 Antibody
Boster
catalog: A00379T117

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: ELISA, immunohistochemistry, immunocytochemistry

quantity: 100 ul
price: 380 USD
to the supplier

Anti-Phospho-Survivin (T34) BIRC5 Antibody
Boster
catalog: A00379T34

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA

quantity: 100 ul
price: 380 USD
to the supplier