Anti-HSP27 Hspb1 Monoclonal Antibody
Boster
catalog: MA1048

mouse monoclonal (IAP-9)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Boster Kit Box
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Hsp27/HSPB1 Antibody Picoband™
Boster
catalog: PB9237

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Anti- HSP27 antibody, PB9237, Western blotting. All lanes: Anti HSP27 (PB9237) at 0.5ug/ml. WB: Recombinant Human HSP27 Protein 0.5ng. Predicted bind size: 19KD. Observed bind size: 19KD

Anti- HSP27 antibody, PB9237, Western blotting. All lanes: Anti HSP27 (PB9237) at 0.5ug/ml. Lane 1: NRK Whole Cell Lysate at 40ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: A549 Whole Cell Lysate at 40ug. Lane 4: COLO320 Whole Cell Lysate at 40ug. Lane 5: HEPG2 Whole Cell Lysate at 40ug. Predicted bind size: 23KD. Observed bind size: 23KD

Anti- HSP27 antibody, PB9237, IHC(P). IHC(P): Human Intestinal Cancer Tissue
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-Phospho-Hsp27 (S78) HSPB1 Rabbit Monoclonal Antibody
Boster
catalog: P00676

domestic rabbit monoclonal (IBC-8)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Western blot analysis of Phospho-Hsp27 (S78) expression in (1) A431 cell lysate; (2) A431 cell lysate treated with Anisomycin (P00676). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB1 monoclonal antibody (Catalog # P00676) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Hsp27/HSPB1 Antibody Picoband™ (monoclonal, 3H3)
Boster
catalog: M00676-5

mouse monoclonal (3H3)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100µg/vial
price: 315 USD
to the supplier

Anti-Phospho-Hsp27 (S82) HSPB1 Rabbit Monoclonal Antibody
Boster
catalog: P00676-1

domestic rabbit monoclonal (FGD-8)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation

Western blot analysis of Phospho-Hsp27 (S82) expression in HeLa cell lysate treated with heat shock (P00676-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB1 monoclonal antibody (Catalog # P00676-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Hsp27 HSPB1 Rabbit Monoclonal Antibody
Boster
catalog: M00676-2

domestic rabbit monoclonal (HII-8)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

Western blot analysis of Hsp27 expression in HeLa cell lysate (M00676-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB1 monoclonal antibody (Catalog # M00676-2) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB1
quantity: 100 µl
price: 315 USD
to the supplier

Anti-Human Hsp27 DyLight® 488 conjugated HSPB1 Antibody(monoclonal, 3H3)
Boster
catalog: M00676-Dyl488

mouse monoclonal (3H3)
reactivity: human
application: flow cytometry

quantity: 50 ug/vial
price: 415 USD
to the supplier