Anti-APE1/APEX1 Antibody
Boster
catalog: PA1494
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Anti-APE1 antibody, PA1494, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: Mouse Brain Tissue Lysate.

Anti-APE1 antibody, PA1494, IHC(P). IHC(P): Rat Intestine Tissue

Anti-APE1 antibody, PA1494, Western blotting. Lane 1: U87 Cell Lysate. Lane 2: A549 Cell Lysate. Lane 3: SMMC Cell Lysate. Lane 4: HELA Cell Lysate.
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-APE1/APEX1 Antibody Picoband™
Boster
catalog: PB9128
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Figure 1. Western blot analysis of APEX1 using anti-APEX1 antibody (PB9128). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human APEX1 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APEX1 antigen affinity purified polyclonal antibody (Catalog # PB9128) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APEX1 at approximately 45KD. The expected band size for APEX1 is at 45KD.

Figure 2. Western blot analysis of APEX1 using anti-APEX1 antibody (PB9128). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: NRK Whole Cell Lysate. Lane 2: HELA Whole Cell Lysate. Lane 3: PC-12 Whole Cell Lysate. Lane 4: RH35 Whole Cell Lysate. Lane 5: HEPA Whole Cell Lysate. Lane 6: MCF Whole Cell Lysate. Lane 7: A549 Whole Cell Lysate. Lane 8: Human Placenta Tissue Lysate. Lane 9: A431 Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APEX1 antigen affinity purified polyclonal antibody (Catalog # PB9128) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APEX1 at approximately 39KD. The expected band size for APEX1 is at 39KD.

Figure 3. IHC analysis of APEX1 using anti-APEX1 antibody (PB9128). APEX1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-APEX1 Antibody (PB9128) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-APE1 APEX1 Antibody Picoband™ (monoclonal, 5C11)
Boster
catalog: M00627
mouse monoclonal (5C11)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

Figure 1. Western blot analysis of APE1 using anti-APE1 antibody (M00627). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, . Lane 2: human MCF-7 whole cell lysates, . Lane 3: human COLO-320 whole cell lysates, . Lane 4: human HepG2 whole cell lysates, . Lane 5: Rabbit IgG, . Lane 6: Marker 1113, . Lane 7: human A549 whole cell lysates , . Lane 8: human PANC-1 whole cell lysates, . Lane 9: human 22RV1 whole cell lysates, . Lane 10: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-APE1 antigen affinity purified monoclonal antibody (Catalog # M00627) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.

Figure 2. IHC analysis of APE1 using anti-APE1 antibody (M00627). APE1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-APE1 Antibody (M00627) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. "
quantity: 100µg/vial
price: 315 USD
to the supplier
Anti-Human APE1 DyLight® 488 conjugated APEX1 Antibody(monoclonal, 5C11)
Boster
catalog: M00627-Dyl488
mouse monoclonal (5C11)
reactivity: human
conjugate: DyLight488
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier
Anti-Human APE1 DyLight® 550 conjugated APEX1 Antibody(monoclonal, 5C11)
Boster
catalog: M00627-Dyl550
mouse monoclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry

Boster Kit Box
quantity: 50 ug/vial
price: 415 USD
to the supplier
Anti-APE1 Rabbit Monoclonal Antibody
Boster
catalog: M00627-3
domestic rabbit monoclonal (17A44)
reactivity: human
application: western blot, immunohistochemistry
quantity: 100 µl/vial
price: 315 USD
to the supplier