domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of GTPase HRAS expression in rat brain extract (lane 1), mouse brain extract (lane 2) and A549 whole cell lysates (lane 3). GTPase HRAS at 21KD was detected using rabbit anti- GTPase HRAS Antigen Affinity purified polyclonal antibody (Catalog # A00114) at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
Anti- GTPase HRAS antibody, RP1098, Western blotting. All lanes: Anti GTPase HRAS (RP1098) at 0.5ug/ml. Lane 1: Mouse Brain Tissue Lysate at 50ug. Lane 2: HELA Whole Cell Lysate at 40ug. Lane 3: A549 Whole Cell Lysate at 40ug. Predicted bind size: 21KD. Observed bind size: 21KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Anti-GTPase HRAS antibody, RP1099, Western blotting. All lanes: Anti GTPase HRAS (RP1099) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: Mouse Brain Tissue Lysate at 50ug. Lane 3: HEPA Whole Cell Lysate at 40ug. Predicted bind size: 21KD. Observed bind size: 21KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (BEA-8)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
Western blot analysis MCF7 cell lysate using HRAS Antibody (M00114). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HRAS monoclonal antibody (Catalog # M00114) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HRAS
quantity: 100 µl
price: 315 USD
to the supplier