domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry
Figure 1. Western blot analysis of GSTM1 using anti-GSTM1 antibody (A00569-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,. Lane 2: rat lung tissue lysates,. Lane 3: mouse stomach tissue lysates,. Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTM1 antigen affinity purified polyclonal antibody (Catalog # A00569-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTM1 at approximately 26KD. The expected band size for GSTM1 is at 26KD.
Figure 2. Flow Cytometry analysis of HeLa cells using anti- GSTM1 antibody (A00569-1). Overlay histogram showing HeLa cells stained with A00569-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GSTM1 Antibody (A00569-1,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
quantity: 100µg/vial
price: 315 USD
to the supplier
mouse monoclonal (11F2)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
Figure 3. IHC analysis of GSTM1 using anti-GSTM1 antibody (M00569). GSTM1 was detected in paraffin-embedded section of rat liver tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml mouse anti-GSTM1 Antibody (M00569) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
quantity: 100µg/vial
price: 315 USD
to the supplier
mouse monoclonal (11F2)
reactivity: human
conjugate: DyLight488
application: flow cytometry
reactivity: human
conjugate: DyLight488
application: flow cytometry
mouse monoclonal
reactivity: human
conjugate: DyLight 550
application: flow cytometry
reactivity: human
conjugate: DyLight 550
application: flow cytometry
domestic rabbit monoclonal (22G11)
reactivity: human
application: western blot
reactivity: human
application: western blot