Anti-PD-L1/B7-H1/CD274 Antibody Picoband™
Boster
catalog: A00109

domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of PD-L1/B7-H1 expression in HELA whole cell lysates (lane 1). PD-L1/B7-H1 at 62KD was detected using rabbit anti- PD-L1/B7-H1 Antigen Affinity purified polyclonal antibody (Catalog # A00109) at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-PD-L1/CD274 Antibody Picoband™
Boster
catalog: PB9154

domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

Anti-PD-L1 Picoband antibody, PB9154-1.jpg. All lanes: Anti PDL1 (PB9154) at 0.5ug/ml. WB: Recombinant Human PDL1 Protein 0.5ng. Predicted bind size: 38KD. Observed bind size: 38KD

Anti-PD-L1 Picoband antibody, PB9154-2.jpg. All lanes: Anti PDL1 (PB9154) at 0.5ug/ml. WB: HEPG2 Whole Cell Lysate at 40ug. Predicted bind size: 33KD . Observed bind size: 33KD
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-PD-L1 (CD274) Rabbit Monoclonal Antibody
Boster
catalog: M00109

domestic rabbit monoclonal (IDC-3)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Western blot analysis of PD-L1 (CD274) expression in Ramos cell lysate (M00109). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD274 monoclonal antibody (Catalog # M00109) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD274

Immunohistochemical analysis of paraffin-embedded human thymoma, using PD-L1 (CD274) Antibody(). PD-L1 (CD274) was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- PD-L1 (CD274) Antibody (M00109) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier

Anti-PD-L1 CD274 Antibody
Boster
catalog: A00109-1

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 0.1 mg
price: 404 USD
to the supplier

Anti-PDL1 CD274 Monoclonal Antibody [4F2]
Boster
catalog: M00109-2

mouse monoclonal (4F2)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 0.1 mg
price: 462 USD
to the supplier

Anti-PDL1 CD274 Monoclonal Antibody [6H10]
Boster
catalog: M00109-3

mouse monoclonal (6H10)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry

quantity: 0.1 mg
price: 462 USD
to the supplier