domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA
Figure 1. Western blot analysis of Angiopoietin-1 using anti-Angiopoietin-1 antibody (A00853). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human ANGPT1 protein 1ng . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin-1 antigen affinity purified polyclonal antibody (Catalog # A00853) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiopoietin-1 at approximately 70KD. The expected band size for Angiopoietin-1 is at 57KD.
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Anti-Angiopoietin 1 Picoband antibody, PB9125-1.jpg. All lanes: Anti Angiopoietin1 (PB9125) at 0.5ug/ml. WB: Recombinant Human Angiopoietin1 Protein 0.5ng. Predicted bind size: 39KD. Observed bind size: 39KD
Anti-Angiopoietin 1 Picoband antibody, PB9125-2.jpg. All lanes: Anti Angiopoietin1 (PB9125) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: MCF-7 Whole Cell Lysate at 40ug. Lane 3: COLO320 Whole Cell Lysate at 40ug. Lane 4: A549 Whole Cell Lysate at 40ug. Lane 5: HEPG2 Whole Cell Lysate at 40ug. Lane 6: 293T Whole Cell Lysate at 40ug. Lane 7: SW620 Whole Cell Lysate at 40ug. Predicted bind size: 57KD. Observed bind size: 65KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (FGC-1)
reactivity: human, rat
application: western blot
reactivity: human, rat
application: western blot
Western blot analysis of Angiopoietin 1 expression in HeLa cell lysate (M00853). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANGPT1 monoclonal antibody (Catalog # M00853) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANGPT1
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
reactivity: human, mouse, rat
application: western blot, immunohistochemistry