domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
Anti-TIM 1 antibody, PA1624, Western blotting. Lane 1: SMMC Cell Lysate . Lane 2: HELA Cell Lysate. Lane 3: PANC Cell Lysate. Lane 4: M231 Cell Lysate. Lane 5: M453 Cell Lysate.
Anti-TIM 1 antibody, PA1624, IHC(P). IHC(P): Human Tonsil Tissue
Anti-TIM 1 antibody, PA1624, ICC. ICC: K562 Cell
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot
reactivity: human
application: western blot
Anti- TIM1 antibody, RP1093, Western blotting. All lanes: Anti TIM1 (RP1093) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: PANC Whole Cell Lysate at 40ug. Lane 3: HEPG2 Whole Cell Lysate at 40ug. Lane 4: A549 Whole Cell Lysate at 40ug. Predicted bind size: 39KD. Observed bind size: 49KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (AFBC-8)
reactivity: human
application: western blot
reactivity: human
application: western blot
Figure 1. Western blot analysis of HAVCR1 using anti-HAVCR1 antibody (M01306). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAVCR1 antigen affinity purified polyclonal antibody (Catalog # M01306) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for HAVCR1.
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA
Figure 1. Western blot analysis of TIM 1 using anti-TIM 1 antibody (A01306). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TIM1 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 1 antigen affinity purified polyclonal antibody (Catalog # A01306) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 1 at approximately 115KD. The expected band size for TIM 1 is at 55KD.
quantity: 100µg/vial
price: 315 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunocytochemistry