Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Antibody (Monoclona ...
Boster
catalog: MA1055

mouse monoclonal (MAPK-YT)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry
citations: 1

Boster Kit Box
quantity: 100 ug/vial
price: 280 USD
to the supplier

Anti-ERK1/MAPK3 Antibody
Boster
catalog: PA1343

rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

Anti-ERK1 antibody, PA1343, Western blotting. Lane 1: Rat Spleen Tissue Lysate. Lane 2: Rat Thymus Tissue Lysate. Lane 3: Rat Skeletal Muscle Tissue Lysate. Lane 4: Rat Kidney Tissue Lysate. Lane 5: HELA Cell Lysate. Lane 6: JURKAT Cell Lysate. Lane 7: RAJI Cell Lysate. Lane 8: COLO320 Cell Lysate

Anti-ERK1 antibody, PA1343, IHC(P). IHC(P): Human Mammary Cancer Tissue

Anti-ERK1 antibody, PA1343, IHC(P). ICC: HELA Cell
quantity: 100 ug/vial
price: 280 USD
to the supplier

Anti-ERK1/2 Rabbit Monoclonal Antibody
Boster
catalog: M00104-1

rabbit monoclonal (DFF-13)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation

Western blot analysis of ERK1/2 Antibody expression in HepG2 whole cell lysates (M00104-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody (Catalog # M00104-1) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK3
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) Rabbit Monoclonal Antibody
Boster
catalog: P00104

rabbit monoclonal (BIH-13)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation

Western blot analysis of Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in A431 cell lysate treated with EGF (P00104). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody (Catalog # P00104) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK3
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-ERK1 Rabbit Monoclonal Antibody
Boster
catalog: M00104

rabbit monoclonal (ADF-13)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ERK1 Antibody(M00104). MAPK3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAPK3 Antibody (M00104)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of ERK1 in NIH-3T3 cell lysate (M00104). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody (Catalog # M00104) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK3
quantity: 100 ug/vial
price: 299 USD
to the supplier

Anti-ERK1 Rabbit Monoclonal Antibody
Boster
catalog: M00104-2

rabbit monoclonal (ACE-13)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human bladder, using ERK1 Antibody(M00104-2). MAPK3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAPK3 Antibody (M00104-2)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of ERK1 in Jurkat cell lysate (M00104-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody (Catalog # M00104-2) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK3
quantity: 100 ug/vial
price: 299 USD
to the supplier