Anti-Fibroblast growth factor 21 FGF21 Antibody
Boster
catalog: PA1673

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Figure 1. Western blot analysis of FGF21 using anti- FGF21 antibody (PA1673). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A549 Whole Cell Lysate,. Lane 2: A431 Whole Cell Lysate,. Lane 3: HEPA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- FGF21 antigen affinity purified polyclonal antibody (Catalog # PA1673) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21 at approximately 21KD. The expected band size for FGF21 is at 21KD.

Figure 2. Western blot analysis of FGF21 using anti- FGF21 antibody (PA1673). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- FGF21 antigen affinity purified polyclonal antibody (Catalog # PA1673) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21 at approximately 21KD. The expected band size for FGF21 is at 21KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-FGF21 Antibody Picoband™
Boster
catalog: PB10063

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA

Figure 1. Western blot analysis of FGF21 using anti-FGF21 antibody (PB10063). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (Catalog # PB10063) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21 at approximately 22KD. The expected band size for FGF21 is at 22KD.

Figure 2. Western blot analysis of FGF21 using anti-FGF21 antibody (PB10063). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, . Lane 2: rat liver tissue lysates, . Lane 3: rat RH35 whole cell lysates, . Lane 4: rat brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (Catalog # PB10063) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21 at approximately 22KD. The expected band size for FGF21 is at 22KD.

Figure 3. Western blot analysis of FGF21 using anti-FGF21 antibody (PB10063). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (Catalog # PB10063) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21 at approximately 22KD. The expected band size for FGF21 is at 22KD.
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-FGF21 Rabbit Monoclonal Antibody
Boster
catalog: M00802

domestic rabbit monoclonal (AG-6)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Western blot analysis of FGF21 expression in (1) HeLa cell lysate; (2) Mouse spleen lysate (M00802). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF21 monoclonal antibody (Catalog # M00802) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21
quantity: 100 µl
price: 315 USD
to the supplier

Anti-FGF21 Antibody Picoband™
Boster
catalog: A00802-1

domestic rabbit polyclonal
reactivity: human
application: ELISA

Boster Kit Box
quantity: 100μg/vial
price: 315 USD
to the supplier

Anti-FGF21 Biotinylated Antibody
Boster
catalog: A00802-Biotin

domestic rabbit polyclonal
reactivity: human
conjugate: biotin
application: ELISA

Boster Kit Box
quantity: 50μg/vial
price: 315 USD
to the supplier

Anti-Fibroblast growth factor 21 FGF21 Antibody
Boster
catalog: A00802

domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunohistochemistry

quantity: 100 ul
price: 697 USD
to the supplier