domestic rabbit polyclonal
reactivity: human, rat
application: western blot
reactivity: human, rat
application: western blot
Anti- GATA3 antibody, PB9199, Western blotting. All lanes: Anti GATA3 (PB9199) at 0.5ug/ml. WB: Recombinant Human GATA3 Protein 0.5ng. Predicted bind size: 39KD. Observed bind size: 39KD
Anti- GATA3 antibody, PB9199, Western blotting. All lanes: Anti GATA3 (PB9199) at 0.5ug/ml. Lane 1: Rat Brain Tissue Lysate at 50ug. Lane 2: MCF-7 Whole Cell Lysate at 40ug. Lane 3: HEPG2 Whole Cell Lysate at 40ug. Predicted bind size: 48KD. Observed bind size: 48KD
quantity: 100μg/vial
price: 315 USD
to the supplier
domestic rabbit monoclonal (ABBH-7)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, chromatin immunoprecipitation
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, chromatin immunoprecipitation
Western blot analysis of GATA3 expression in (1) SH-SY5Y cell lysate; (2) RAW 2647 cell lysate (M00593). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 monoclonal antibody (Catalog # M00593) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA3
IHC analysis of GATA3 using anti-GATA3 antibody (M00593) on human bladder cancer. GATA3 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GATA3 Antibody (M00593) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit monoclonal (DHF-7)
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
reactivity: human
application: western blot, immunohistochemistry, immunoprecipitation
Immunohistochemical analysis of paraffin-embedded human bladder cancer, using Phospho-GATA3 (S308) Antibody(P00593). GATA3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GATA3 Antibody (P00593)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of Phospho-GATA3 (S308) Jurkat cell lysate treated with Bromo-cAMP (P00593). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 monoclonal antibody (Catalog # P00593) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA3
quantity: 100 µl
price: 315 USD
to the supplier
domestic rabbit polyclonal (polyclonal)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section