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> Boster Flt-1 antibody
Anti-VEGF Receptor 1/FLT1 Antibody
Boster
catalog: PA1399
domestic rabbit polyclonal
reactivity:
human
application:
western blot
Anti-FLT1 antibody, PA1399, Western blotting. Lane 1: MCF-7 Cell Lysate. Lane 2: SGC Cell Lysate. Lane 3: MM231 Cell Lysate.
quantity: 100μg/vial
price: 315 USD
to the supplier
Anti-VEGF Receptor 1 FLT1 Rabbit Monoclonal Antibody
Boster
catalog: M00534
domestic rabbit monoclonal (AFH-6)
reactivity:
human
,
mouse
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
,
immunoprecipitation
,
flow cytometry
Western blot analysis of VEGFR1 expression in A431 cell lysate (M00534). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLT1 monoclonal antibody (Catalog # M00534) overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLT1
quantity: 100 µl
price: 315 USD
to the supplier
Anti-VEGF Receptor 1/FLT1 Antibody Picoband™
Boster
catalog: A00534
domestic rabbit polyclonal
reactivity:
human
,
mouse
application:
western blot
,
ELISA
,
immunocytochemistry
,
flow cytometry
Figure 1. Western blot analysis of VEGF Receptor 1 using anti-VEGF Receptor 1 antibody (A00534). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate,. Lane 2: human placenta tissue lysate,. Lane 3: human A549 cell lysate,. Lane 4: human COLO-320 cell lysate,. Lane 5: Human A431 cell lysate,. Lane 6: human SK-OV-3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF Receptor 1 antigen affinity purified polyclonal antibody (Catalog # A00534) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF Receptor 1 at approximately 125KD. The expected band size for VEGF Receptor 1 is at 150KD.
quantity: 100µg/vial
price: 315 USD
to the supplier
Anti-VEGF Receptor 1/FLT1 Antibody Picoband™
Boster
catalog: A00534-1
domestic rabbit polyclonal
reactivity:
human
,
mouse
application:
western blot
Figure 1. Western blot analysis of VEGF Receptor 1 using anti-VEGF Receptor 1 antibody (A00534-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate,. Lane 2: mouse liver tissue lysate,. Lane 3: mouse HEPA1-6 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF Receptor 1 antigen affinity purified polyclonal antibody (Catalog # A00534-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF Receptor 1 at approximately 150KD. The expected band size for VEGF Receptor 1 is at 150KD.
quantity: 100µg/vial
price: 315 USD
to the supplier
Anti-VEGFR1 FLT1 Antibody
Boster
catalog: A00534-3
domestic rabbit polyclonal (polyclonal)
reactivity:
human
application:
western blot
,
immunohistochemistry
,
immunocytochemistry
,
immunoprecipitation
,
flow cytometry
quantity: 100 ul
price: 697 USD
to the supplier
Anti-FLT1 Antibody Picoband™
Boster
catalog: A00534-4
domestic rabbit polyclonal (polyclonal)
reactivity:
human
application:
western blot
,
ELISA
,
immunocytochemistry
,
flow cytometry
quantity: 100µg/vial
price: 315 USD
to the supplier
Anti-Phospho-Flt-1 (Y1048) Antibody
Boster
catalog: A00534Y1048
domestic rabbit polyclonal (polyclonal)
reactivity:
human
application:
ELISA
,
immunohistochemistry
quantity: 100 ul
price: 380 USD
to the supplier
Anti-Phospho-Flt-1 (Y1213) Antibody
Boster
catalog: A00534Y1213
domestic rabbit polyclonal (polyclonal)
reactivity:
human
application:
western blot
,
ELISA
quantity: 100 ul
price: 380 USD
to the supplier
Anti-Phospho-Flt-1 (Y1333) Antibody
Boster
catalog: A00534Y1333-1
domestic rabbit polyclonal (polyclonal)
reactivity:
human
application:
western blot
,
ELISA
,
immunohistochemistry
quantity: 100 ul
price: 380 USD
to the supplier
Anti-FLT1 Antibody
Boster
catalog: M00534-1
mouse monoclonal (monoclonal)
reactivity:
human
application:
western blot
quantity: 80 ul
price: 170 USD
to the supplier